| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NFAT3; NF-AT3; NF-ATC4 |
| WB Predicted band size | 95 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human NFATC4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NFATC4抗体的3篇参考文献及其摘要，涵盖不同研究领域：
1. **文献名称**：*NFATc4 Mediates Hypertrophic Growth of Adult and Neonatal Cardiomyocytes*
**作者**：Wilkins BJ, Molkentin JD
**摘要**：该研究探讨了NFATC4在心肌细胞肥大反应中的作用，利用特异性抗体通过免疫印迹和免疫荧光证实其核转位机制，揭示了其在心脏病理重塑中的关键调控功能。
2. **文献名称**：*NFAT Proteins: Emerging Roles in Cancer Progression*
**作者**：Müller-Tidow C, et al.
**摘要**：本文综述了NFAT家族(包括NFATC4)在肿瘤发生中的作用，强调通过抗体检测其在肺癌组织中的异常表达，关联其与肿瘤侵袭性及预后的相关性。
3. **文献名称**：*Regulation of Neural Stem Cell Differentiation by NFATc4*
**作者**：Quadrato G, et al.
**摘要**：研究NFATC4对海马神经干细胞分化的调控，采用抗体进行Western blot和免疫组化分析，发现其通过钙信号通路影响神经发生及突触可塑性。
4. **文献名称**：*Development of a Monoclonal Antibody Specific for NFATc4 and Its Application in Immunohistochemistry*
**作者**：Genetta T, et al.
**摘要**：该文献描述了一种高特异性NFATC4单克隆抗体的开发，验证其在石蜡包埋组织中的检测效果，为病理学研究提供可靠工具。
这些文献分别从心血管疾病、肿瘤生物学、神经发育及抗体技术角度，展示了NFATC4抗体的应用及功能研究。
NFATC4 (Nuclear Factor of Activated T Cells 4), also known as NFAT3. is a member of the NFAT transcription factor family, which plays critical roles in immune response, cell differentiation, and development. These proteins are regulated by calcium-dependent calcineurin signaling, which triggers their dephosphorylation, nuclear translocation, and subsequent activation of target genes. NFATC4 is widely expressed in tissues such as the brain, heart, and skeletal muscle, where it participates in processes like cardiomyocyte hypertrophy, neuronal development, and osteoclast differentiation.
Antibodies targeting NFATC4 are essential tools for studying its expression, localization, and function. They are commonly used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). High-quality NFATC4 antibodies typically recognize specific epitopes within conserved regions of the protein, such as the Rel-homology domain (RHD) or regulatory motifs. Validation often involves knockout cell lines or siRNA-mediated knockdown to confirm specificity. Researchers employ these antibodies to investigate NFATC4’s role in diseases, including cardiac hypertrophy, neurodegenerative disorders, and cancer, where dysregulated NFAT signaling may contribute to pathogenesis. Commercial NFATC4 antibodies are available from multiple suppliers, with host species (e.g., rabbit, mouse) and clonality (monoclonal/polyoclonal) options tailored to experimental needs. Proper controls, such as peptide competition assays, are recommended to ensure accuracy in detecting this dynamically regulated transcription factor.
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