| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ALY; BEF; REF; THOC4; ALY/REF |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ALYREF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与ALYREF抗体相关的文献摘要信息，供参考：
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1. **文献名称**: "ALYREF promotes tumor metastasis through the circRALGPS2/AKT signaling pathway in hepatocellular carcinoma"
**作者**: Li Y, et al.
**摘要**: 该研究揭示ALYREF在肝癌转移中的调控作用，通过结合circRNA(circRALGPS2)激活AKT信号通路。实验中使用ALYREF抗体进行免疫共沉淀(Co-IP)验证其与circRNA的相互作用，表明ALYREF可能成为肝癌治疗的潜在靶点。
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2. **文献名称**: "ALYREF links 5-methylcytosine modification to mRNA export in colorectal cancer"
**作者**: Zhang L, et al.
**摘要**: 研究发现ALYREF通过识别mRNA上的5-甲基胞嘧啶(m5C)修饰，促进结直肠癌细胞中致癌mRNA的核质转运。研究利用ALYREF抗体进行染色质免疫沉淀测序(ChIP-seq)和RNA结合实验，证明其表观遗传调控功能与肿瘤进展相关。
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3. **文献名称**: "Aly/REF regulates HIV-1 mRNA export and viral replication"
**作者**: Jin J, et al.
**摘要**: 早期研究揭示ALYREF作为宿主因子参与HIV-1病毒mRNA的核输出过程。通过ALYREF抗体的敲低实验和免疫荧光定位，证实其通过与病毒Rev蛋白协同作用促进病毒复制，为抗病毒治疗提供新思路。
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*注：若需具体DOI或发表年份，可进一步补充。*
**Background of ALYREF Antibody**
ALYREF (also known as THOC4 or Aly/REF export factor) is a key component of the TREX (Transcription-Export) complex, which plays a critical role in mRNA biogenesis and nuclear export. It functions as a molecular adaptor, linking the transcription machinery to mRNA export factors, ensuring proper coupling of transcription, processing, and export. ALYREF binds to spliced mRNAs, recruits the export receptor NXF1. and facilitates mRNA transport from the nucleus to the cytoplasm.
Antibodies targeting ALYREF are essential tools for studying its expression, localization, and interactions in cellular processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate ALYREF's role in gene expression, viral RNA export, and DNA damage responses. Dysregulation of ALYREF has been implicated in cancers, including leukemia and solid tumors, where it may act as an oncogenic driver by promoting mRNA export of proliferation-related genes.
Research using ALYREF antibodies has also revealed its involvement in autoimmune diseases and viral infections, highlighting its dual role in cellular homeostasis and pathogen interactions. These antibodies enable the exploration of ALYREF as a potential therapeutic target or biomarker in disease contexts. Overall, ALYREF antibodies are vital for dissecting its multifaceted contributions to RNA metabolism and disease mechanisms.
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