| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | NEM2, NEB177D |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NEB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NEB抗体的3篇参考文献的概括(虚构示例，仅供参考)：
1. **文献名称**：*"High-specificity monoclonal antibodies against NEB-derived epitopes for neuromuscular disease diagnostics"*
**作者**：Thompson, R. et al.
**摘要**：本研究开发了一组针对NEB(nebulin)蛋白C端表位的高特异性单克隆抗体，验证了其在免疫组化和Western blot中的应用。结果显示，这些抗体能够有效区分正常与nemaline肌病患者的肌肉组织样本，为临床诊断提供了新工具。
2. **文献名称**：*"NEB-supplied CRISPR/Cas9 antibodies enable precise off-target detection in gene-edited cells"*
**作者**：Zhang, L. et al.
**摘要**：通过使用NEB公司生产的Anti-Cas9抗体，研究者建立了灵敏的ELISA检测方法，用于定量分析CRISPR编辑细胞中Cas9蛋白的表达水平及脱靶效应。该抗体在低浓度下仍表现出高亲和力，显著提升了检测的准确性。
3. **文献名称**：*"A novel polyclonal antibody targeting NEB mutations in congenital myopathy models"*
**作者**：Ito, S. et al.
**摘要**：研究团队利用重组NEB突变蛋白免疫动物，制备出多克隆抗体，成功在小鼠模型中定位了NEB基因突变导致的肌纤维结构异常。该抗体为研究先天性肌无力的病理机制提供了关键工具。
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**注**：以上文献为模拟内容，实际文献需通过数据库(如PubMed)检索关键词“nebulin antibody”或“NEB antibody”获取。若需真实文献，建议补充具体研究方向(如疾病或技术应用)。
**Background of NEB Antibodies**
New England Biolabs (NEB), established in 1974. is a globally recognized leader in the production of reagents for molecular biology, including high-quality antibodies. NEB antibodies are widely utilized in research for their reliability and specificity, supporting applications such as Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and epigenetic studies.
NEB’s antibody development emphasizes rigorous validation to ensure minimal batch-to-batch variability, a common challenge in antibody production. Their portfolio includes antibodies targeting post-translational modifications (e.g., phosphorylation, acetylation, methylation), histone modifications, and key signaling proteins, which are critical for studying cellular processes and disease mechanisms.
A distinguishing feature of NEB antibodies is their integration with recombinant protein technology. Many are produced using recombinant methods, enhancing consistency and reducing reliance on animal-based production. This approach aligns with evolving ethical standards and improves reproducibility in research.
Additionally, NEB provides detailed validation data, including application-specific protocols and peer-reviewed references, ensuring researchers can confidently incorporate these tools into experimental workflows. By combining technical innovation with a commitment to quality, NEB antibodies contribute to advancing molecular and cellular biology research, enabling discoveries in areas such as cancer, epigenetics, and signal transduction.
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