| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ACN; ACINUS; fSAP152 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ACIN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ACIN1抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*"Acinus promotes apoptotic chromatin condensation through a caspase-3-dependent mechanism"*
**作者**：Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y
**摘要**：该研究揭示了ACIN1在细胞凋亡过程中通过caspase-3依赖的机制促进染色质凝聚的作用，研究中使用ACIN1抗体通过Western blot和免疫荧光验证蛋白表达及定位。
2. **文献名称**：*"The human RNA-binding protein Acinus interacts with caspase-3 and is involved in apoptosis"*
**作者**：Zhou Q, Li Z, Li W
**摘要**：文章探讨了ACIN1在凋亡中与caspase-3的相互作用，利用特异性ACIN1抗体进行免疫共沉淀(Co-IP)和蛋白质组学分析，证实其在凋亡信号通路中的功能。
3. **文献名称**：*"Proteomic analysis of the human nuclear pore complex interactome reveals a role for Acinus in mRNA splicing"*
**作者**：Beliak-Bakke K, Pendleton CS, Kehlenbach RH
**摘要**：研究通过质谱和ACIN1抗体介导的免疫沉淀，发现ACIN1不仅参与凋亡，还与核孔复合物及mRNA剪接复合体存在关联，扩展了其功能认知。
4. **文献名称**：*"Subcellular localization and phosphorylation of Acinus during apoptosis"*
**作者**：Kadota S, Shirayoshi Y
**摘要**：该文献利用ACIN1抗体进行亚细胞定位研究，发现其磷酸化状态在凋亡过程中发生变化，并影响其在核内的聚集及功能。
以上文献均涉及ACIN1抗体的实验应用(如Western blot、免疫荧光、Co-IP等)，并围绕其凋亡或RNA代谢功能展开。
ACIN1 (Apoptotic Chromatin Condensation Inducer 1) is a nuclear protein involved in chromatin condensation and nuclear fragmentation during apoptosis. It was initially identified as a substrate for caspase-3. with cleavage required to activate its pro-apoptotic function. Structurally, ACIN1 contains an N-terminal nuclear localization signal, a central RNA recognition motif (RRM), and a C-terminal proline-rich domain. Beyond apoptosis, ACIN1 plays roles in RNA processing, particularly in alternative splicing, through its interaction with components of the spliceosome complex like SAP18.
Antibodies targeting ACIN1 are widely used in research to study its dual roles in cell death and RNA metabolism. These tools enable detection of full-length ACIN1 (~150 kDa) and its caspase-cleaved fragments (~65-85 kDa) in apoptosis assays. Commercial antibodies are typically validated for applications including Western blotting, immunofluorescence, and immunoprecipitation. However, users should note potential cross-reactivity with splice variants (e.g., ACIN1B) and consider post-translational modifications (e.g., phosphorylation) that may affect migration patterns in SDS-PAGE. Recent studies also explore ACIN1's involvement in neurological disorders and cancer progression, expanding its research relevance.
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