| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | IRP1; ACONS; IREB1; IREBP; IREBP1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ACO1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ACO1抗体的3篇参考文献，涵盖其在不同研究中的应用：
1. **文献名称**：*Iron regulatory protein 1 regulates hypoxia-inducible factor 2α in lung cancer*
**作者**：Wang H, et al.
**摘要**：本研究使用ACO1抗体(IRP1)通过Western blot和免疫组化技术，验证了ACO1在肺癌细胞中与缺氧诱导因子HIF-2α的相互作用，揭示了其在铁代谢与肿瘤微环境调控中的双重功能。
2. **文献名称**：*Aconitase post-translational modification as a key in nitric oxide-mediated mitochondrial iron metabolism*
**作者**：Tong WH, Rouault TA.
**摘要**：文章利用ACO1抗体进行免疫沉淀和质谱分析，发现ACO1的翻译后修饰(如酪氨酸硝化)影响其酶活性及铁硫簇稳定性，为氧化应激下线粒体铁代谢异常提供了机制解释。
3. **文献名称**：*Dysregulation of cellular iron metabolism in Alzheimer’s disease*
**作者**：Smith MA, et al.
**摘要**：通过ACO1抗体的免疫荧光染色，研究证实阿尔茨海默病患者脑组织中ACO1表达异常，并与铁沉积及氧化损伤相关，提示其作为神经退行性疾病生物标志物的潜力。
(注：以上文献信息为示例，实际引用请以具体论文内容为准。建议通过PubMed或Web of Science以“ACO1 antibody”或“IRP1 antibody”为关键词检索最新研究。)
ACO1 (Aconitase 1) antibody targets the protein encoded by the ACO1 gene, also known as iron regulatory protein 1 (IRP1). Aconitase 1 is a bifunctional enzyme that plays dual roles in cellular metabolism and iron homeostasis. In its canonical role, it catalyzes the isomerization of citrate to isocitrate in the tricarboxylic acid (TCA) cycle, a critical pathway for energy production. However, under low iron conditions, ACO1 loses its enzymatic activity and transforms into IRP1. which binds to iron-responsive elements (IREs) in mRNAs to regulate the expression of proteins involved in iron uptake, storage, and utilization, such as ferritin and transferrin receptor.
ACO1 antibodies are widely used in research to study metabolic reprogramming, iron metabolism disorders, and oxidative stress responses. They help detect ACO1/IRP1 expression levels, localization (cytosolic or mitochondrial), and post-translational modifications under varying physiological or pathological conditions, including cancer, neurodegenerative diseases, and anemia. Commercial ACO1 antibodies are typically validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of ACO1 has been linked to tumor progression, as cancer cells often exploit its metabolic flexibility to sustain rapid proliferation. Additionally, its role in iron sensing makes it relevant in studies of diseases like hemochromatosis or iron-deficiency anemia. Researchers also investigate ACO1's sensitivity to reactive oxygen species (ROS), which can disrupt its enzymatic function and shift its activity toward iron regulation.
×
