| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | PFK-2/FBPase-2 |
| WB Predicted band size | 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human PFKFB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PFKFB2抗体的3篇文献摘要示例(注：文献为示例性质，可能非真实存在)：
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1. **文献名称**：*Development of a High-Affinity Monoclonal Antibody for PFKFB2 and Its Application in Metabolic Studies*
**作者**：Lee, J. et al.
**摘要**：该研究报道了一种针对PFKFB2蛋白的高特异性单克隆抗体的开发，通过Western blot和免疫荧光验证其结合能力，并应用于肿瘤细胞代谢分析，发现PFKFB2在糖酵解通路的调控中起关键作用。
2. **文献名称**：*PFKFB2 Expression Correlates with Poor Prognosis in Glioblastoma: An Immunohistochemical Study*
**作者**：Smith, R. et al.
**摘要**：利用PFKFB2抗体对胶质母细胞瘤组织进行免疫组化分析，发现PFKFB2高表达与患者生存期缩短显著相关，提示其可作为潜在预后标志物及治疗靶点。
3. **文献名称**：*Mechanistic Insights into PFKFB2-Mediated Cardiac Hypertrophy Using Antibody-Based Detection*
**作者**：Wang, Y. et al.
**摘要**：通过PFKFB2抗体研究其在心肌细胞中的表达定位，发现压力超负荷模型中PFKFB2通过调节果糖-2.6-二磷酸水平促进病理性心肌肥厚，为心脏代谢干预提供依据。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science以关键词“PFKFB2 antibody”或“PFKFB2 immunodetection”检索最新成果，并优先选择经过抗体验证(如KO验证、应用多技术平台)的高影响力论文。
The PFKFB2 antibody is a crucial tool in studying the 6-phosphofructo-2-kinase/fructose-2.6-biphosphatase 2 (PFKFB2) protein, a member of the PFKFB enzyme family. PFKFB enzymes regulate glycolysis by controlling levels of fructose-2.6-bisphosphate (Fru-2.6-BP), a potent allosteric activator of phosphofructokinase-1 (PFK-1), a rate-limiting glycolytic enzyme. Unlike other isoforms (e.g., PFKFB3), PFKFB2 primarily functions as a kinase, promoting Fru-2.6-BP synthesis, but also exhibits phosphatase activity, balancing glycolytic flux. It is expressed in tissues like the liver, muscle, and heart, and plays roles in metabolic homeostasis, cell cycle regulation, and apoptosis.
PFKFB2 antibodies are widely used in research to detect protein expression, localization (cytoplasmic/nuclear), and post-translational modifications via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Studies highlight its involvement in diseases, particularly cancer, where altered PFKFB2 expression correlates with tumor progression and metabolic reprogramming. For example, elevated PFKFB2 in certain cancers may enhance glycolysis, supporting rapid proliferation. Conversely, its downregulation in others suggests context-dependent roles. The antibody’s specificity is validated using knockdown/knockout controls, ensuring reliable detection. Recent research also explores PFKFB2’s interaction with signaling pathways (e.g., AMPK, HIF-1α) and potential therapeutic targeting. Overall, PFKFB2 antibodies are vital for unraveling metabolic adaptations in health and disease.
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