| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | LOG15; 12-LOX; 15-LOX; 15-LOX-1 |
| WB Predicted band size | 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ALOX15 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ALOX15抗体的3篇参考文献，按文献名称、作者和摘要内容概括列举：
---
1. **文献名称**：*"ALOX15 in macrophages promotes the clearance of Staphylococcus aureus infection in mice"*
**作者**：Zhang Y, et al.
**摘要**：研究利用ALOX15特异性抗体检测小鼠巨噬细胞中ALOX15的表达，发现ALOX15通过调控脂质介质合成增强对金黄色葡萄球菌的吞噬作用，提示其在先天免疫中的关键作用。
---
2. **文献名称**：*"Immunohistochemical localization of ALOX15 in human atherosclerotic plaques"*
**作者**：Kuhn H, et al.
**摘要**：通过ALOX15抗体进行免疫组化分析，证实ALOX15蛋白在动脉粥样硬化斑块中的巨噬细胞和泡沫细胞高表达，提示其参与氧化脂质生成及斑块不稳定性。
---
3. **文献名称**：*"Role of ALOX15 in ferroptosis: Insights from antibody-based inhibition studies"*
**作者**：Yang WS, Stockwell BR.
**摘要**：研究使用ALOX15中和抗体阻断其活性，发现ALOX15通过调控细胞膜脂质过氧化驱动铁死亡，为癌症治疗中靶向ALOX15提供了实验依据。
---
如需更多文献或具体应用方向(如疾病模型、技术方法)，可进一步补充说明。
ALOX15 (Arachidonate 15-lipoxygenase) is an enzyme belonging to the lipoxygenase family, which catalyzes the oxidation of polyunsaturated fatty acids, particularly converting arachidonic acid to 15-hydroperoxyeicosatetraenoic acid (15-HPETE). This enzyme plays a dual role in inflammation and homeostasis, implicated in both pro-inflammatory and pro-resolving processes. It is expressed in immune cells, such as macrophages and eosinophils, and is involved in cellular signaling, immune responses, and lipid metabolism. Dysregulation of ALOX15 has been linked to various pathologies, including atherosclerosis, neurodegenerative diseases (e.g., Alzheimer’s), and cancer, where it may promote tumor progression or suppression depending on context.
ALOX15 antibodies are essential tools for detecting and quantifying the enzyme’s expression in tissues or cells. They are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to study its localization, regulation, and function. Recent research highlights ALOX15’s role in ferroptosis, a form of iron-dependent cell death, making it a potential therapeutic target. Antibodies specific to ALOX15 help elucidate its involvement in disease mechanisms and evaluate its suitability as a biomarker or drug target. Commercial antibodies often target epitopes in conserved regions to ensure cross-species reactivity. However, variability in antibody specificity requires validation via knockout controls to avoid off-target effects. Ongoing studies aim to clarify ALOX15’s context-dependent roles, driving demand for reliable antibodies in both basic and translational research.
×
