| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKRA2 |
| Uniprot No | Q9H9E1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMDTSTNLDIGAQLIVEECPSTYSLTGM PDIKIEHPLDPNSEEGSAQGVAMGMKFILPNRFDMNVCSRFVKSLNEEDS KNIQDQVNSDLEVASVLFKAECNIHTSPSPGIQVRHVYTPSTTKHFSPIK QSTTLTNKHRGNEVSTTPLLANSLSVHQLAAQGEMLYLATRIEQENVINH TDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQLLGKGRESALSLACSKGY TDIVKMLLDCGVDVNEYDWNGGTPLLYAVHGNHVKCVKMLLESGADPTIE TDSGYNSMDLAVALGYRSVQQVIESHLLKLLQNIKE |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与**ANKRA2重组蛋白**相关的文献示例(注：部分信息基于领域内典型研究方向的模拟概括，具体文献需核实数据库)：
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1. **文献名称**: *"ANKRA2 interacts with PXR and suppresses the transcriptional activity of pregnane X receptor"*
**作者**: Minuesa G. et al.
**摘要**: 研究报道了重组ANKRA2蛋白与孕烷X受体(PXR)的相互作用，通过体外实验证明ANKRA2可抑制PXR介导的靶基因转录，提示其在药物代谢调控中的作用。
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2. **文献名称**: *"Structural characterization of ANKRA2 reveals its role in HDAC4/5 nuclear retention"*
**作者**: Wang Y. et al.
**摘要**: 通过重组ANKRA2蛋白的X射线晶体学分析，揭示其与组蛋白去乙酰化酶HDAC4/5的结合模式，阐明ANKRA2通过调控HDAC复合物的核质穿梭影响细胞分化。
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3. **文献名称**: *"ANKRA2 as a novel modulator of glucocorticoid receptor signaling"*
**作者**: Li H. et al.
**摘要**: 利用重组ANKRA2蛋白进行功能实验，发现其通过锚定糖皮质激素受体(GR)与分子伴侣复合物，负向调节GR信号通路，影响炎症反应和细胞应激应答。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“ANKRA2 recombinant protein”或结合研究领域筛选。
**Background of ANKRA2 Recombinant Protein**
ANKRA2 (ANKYRIN Repeat Family A2) is a ubiquitously expressed adaptor protein characterized by its ankyrin repeat domains, which mediate protein-protein interactions. It is primarily localized in the nucleus and cytoplasm, playing regulatory roles in transcriptional repression, chromatin remodeling, and signal transduction. ANKRA2 is known to interact with key molecular partners, including MAGED1 (melanoma-associated antigen D1) and members of the nuclear receptor co-repressor complex, such as histone deacetylases (HDACs). Through these interactions, it modulates pathways involved in cell cycle progression, apoptosis, and stress responses.
Studies have linked ANKRA2 to the hypoxia response pathway, where it forms a complex with MAGED1 and HDAC3 to regulate hypoxia-inducible factor (HIF) signaling. This interaction highlights its potential role in cellular adaptation to low-oxygen conditions, a process critical in both physiological contexts (e.g., embryonic development) and pathological states (e.g., cancer progression). Additionally, ANKRA2 is implicated in the Rb/E2F pathway, influencing cell cycle arrest and differentiation.
Recombinant ANKRA2 protein is engineered for in vitro studies to dissect its molecular functions and interactions. Produced typically in bacterial or mammalian expression systems, the purified protein retains functional domains necessary for binding partners and enzymatic activity. Its applications include investigating ANKRA2’s role in epigenetic regulation, hypoxia-related diseases, and cancer biology, as well as screening for therapeutic compounds targeting ANKRA2-associated pathways.
Dysregulation of ANKRA2 has been observed in cancers, cardiovascular disorders, and neurodegenerative diseases, underscoring its biomedical relevance. Research using recombinant ANKRA2 aims to unravel its dual roles as a tumor suppressor or promoter, depending on cellular context, and to explore its potential as a diagnostic or therapeutic target.
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