| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | D2; 5DII; SelY; DIOII; TXDI2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human DIO2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DIO2抗体的参考文献示例(内容为模拟，建议通过学术数据库验证)：
1. **文献名称**: *"DIO2 Antibody Validation in Human Thyroid Disorders"*
**作者**: Martinez et al. (2020)
**摘要**: 该研究通过Western blot和免疫组化验证了DIO2抗体的特异性，发现甲状腺功能亢进患者中DIO2蛋白表达显著升高，提示其在甲状腺激素代谢中的关键作用。
2. **文献名称**: *"DIO2 Expression in Adipose Tissue: Implications for Obesity"*
**作者**: Silva et al. (2018)
**摘要**: 利用DIO2抗体检测肥胖小鼠模型的脂肪组织，发现DIO2水平与能量代谢相关基因表达呈正相关，表明其可能通过调节局部T3水平影响脂质代谢。
3. **文献名称**: *"Developmental Regulation of DIO2 in the Mammalian Brain"*
**作者**: Nguyen & Larsen (2017)
**摘要**: 通过免疫荧光染色结合DIO2抗体，研究揭示了DIO2在小鼠大脑发育不同阶段的动态表达，提示其在神经分化和突触形成中的潜在作用。
4. **文献名称**: *"DIO2 Gene Polymorphism and Protein Localization in Human Placenta"*
**作者**: Wang et al. (2021)
**摘要**: 采用DIO2特异性抗体分析胎盘组织，发现特定基因多态性与DIO2蛋白表达降低相关，可能影响妊娠期母体甲状腺激素稳态。
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**建议**：以上为模拟示例，实际文献请通过PubMed或Google Scholar检索关键词(如“DIO2 antibody”、“Type 2 deiodinase immunohistochemistry”)获取最新研究。真实文献可能包括经典研究(如Bianco AC团队)或近年应用(如代谢疾病、神经科学领域)。
DIO2 (iodothyronine deiodinase type 2) is a critical enzyme in thyroid hormone metabolism, responsible for converting the prohormone thyroxine (T4) into the biologically active triiodothyronine (T3). This selenoprotein is expressed in tissues such as the thyroid, brain, brown adipose tissue, and placenta, where localized T3 production regulates metabolic processes, thermogenesis, and neurodevelopment. DIO2 activity is tightly regulated by thyroid hormone levels, hypoxia, and circadian rhythms, making it pivotal in maintaining tissue-specific hormone homeostasis.
Antibodies targeting DIO2 are essential tools for studying its expression, localization, and function. They enable researchers to detect DIO2 protein levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Such studies have revealed DIO2's role in adaptive thermogenesis, energy balance, and pathological conditions including hypothyroidism, metabolic syndrome, and neuropsychiatric disorders. For instance, DIO2 dysfunction has been linked to insulin resistance and altered hypothalamic-pituitary-thyroid axis feedback.
Commercial DIO2 antibodies are typically raised against specific epitopes, with validation in knockout models to ensure specificity. Challenges include cross-reactivity with other deiodinases (DIO1/DIO3) due to structural similarities. Recent applications extend to exploring DIO2 polymorphisms in clinical cohorts and its interplay with circadian clock proteins. Ongoing research leverages these antibodies to dissect DIO2's tissue-specific roles and therapeutic potential in thyroid-related pathologies.
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