首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human Complement 3,C3 ELISA Kit

  • 中文名： 人补体蛋白3(C3)ELISA Kit
货号: IPD-E08665h
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    人补体蛋白3(C3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、唾液样本中的C3含量。C3作为靶点在生物医学领域有重要意义。它是补体系统关键成分，在免疫防御、炎症反应等生理病理过程中发挥作用。研究显示通过调控C3活性可干预相关疾病进展，如某些自身免疫病和肾脏疾病，其机制研究为新药研发提供方向。试剂盒检测范围为4.7 ng/mL-300 ng/mL，灵敏度为4.031 ng/mL。为科研领域研究补体系统激活机制、炎症相关疾病模型构建、药物干预效果评估及生物标志物探索提供可靠工具，尤其适合实验室进行免疫微环境分析或跨物种比较研究中的C3蛋白定量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    ASP ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 ELISA Kit; C3 ELISA Kit; C3adesArg ELISA Kit; C3bc ELISA Kit; CO3_HUMAN ELISA Kit; Complement C3c alpha'' chain fragment 2 ELISA Kit
  • 缩写：
    C3
  • Uniprot No.：
    P01024
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, urine, saliva
  • 检测范围：
    4.7 ng/mL-300 ng/mL
  • 灵敏度：
    4.031 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.


  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.



  • These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human C3 antibody.

    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial HRP-conjugated C3 antibody(100 x concentrate)(120 μl/bottle) --- Bind to the C3 in the samples or standards and react with the substrate to make the solution chromogenic.

    • One vial Wash Buffer (25x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.

    • One TMBSubstrate (10 ml/bottle) ---Interact with HRP to elicit a chromogenic reaction.

    • One vial Stop Solution (10ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days


      客户数据及评论

      折叠内容

      大包装询价

      ×