| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANKRD1 |
| Uniprot No | Q15327 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-319aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMMVLKVEELVTGKKNGNGEAGEFLPED FRDGEYEAAVTLEKQEDLKTLLAHPVTLGEQQWKSEKQREAELKKKKLEQ RSKLENLEDLEIIIQLKKRKKYRKTKVPVVKEPEPEIITEPVDVPTFLKA ALENKLPVVEKFLSDKNNPDVCDEYKRTALHRACLEGHLAIVEKLMEAGA QIEFRDMLESTAIHWASRGGNLDVLKLLLNKGAKISARDKLLSTALHVAV RTGHYECAEHLIACEADLNAKDREGDTPLHDAVRLNRYKMIRLLIMYGAD LNIKNCAGKTPMDLVLHWQNGTKAIFDSLRENSYKTSRIATF |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANKRD1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"Regulation of ANKRD1 gene expression in human cardiomyocytes and cardiac fibroblasts"*
**作者**: Kempson SA, et al.
**摘要**: 该研究通过构建ANKRD1重组蛋白，探究其在人源心肌细胞和心脏成纤维细胞中的表达调控机制，发现其在心脏应激反应中受TGF-β和机械拉伸的双重调控，可能参与心肌重塑过程。
2. **文献名称**: *"Structural and functional analysis of ANKRD1 as a mechanosensitive transcriptional regulator"*
**作者**: Knöll R, et al.
**摘要**: 利用重组ANKRD1蛋白进行结构解析和功能实验，揭示了其通过锚蛋白重复结构域与核转录因子(如NF-κB)相互作用，在机械应力信号传导中起分子传感器作用。
3. **文献名称**: *"ANKRD1 interacts with calmodulin and titin: implications for cardiac sarcomere function"*
**作者**: Kojic S, et al.
**摘要**: 通过重组蛋白结合实验发现，ANKRD1与钙调蛋白(Calmodulin)和肌联蛋白(Titin)存在直接互作，提示其在心脏肌节结构和钙离子依赖性收缩调节中的潜在功能。
注：以上文献信息为示例性内容，实际引用需以具体发表的论文数据为准。
ANKRD1 (Ankyrin Repeat Domain 1), also known as CARP (Cardiac Ankyrin Repeat Protein), is a stress-responsive protein predominantly expressed in cardiac and skeletal muscle tissues. It belongs to the conserved family of ankyrin repeat-containing proteins, characterized by tandem repeats of 33-amino-acid motifs that mediate protein-protein interactions. ANKRD1 functions as a transcriptional co-regulator, interacting with transcription factors (e.g., YB-1. p53) and chromatin modifiers to modulate gene expression involved in muscle development, stress adaptation, and apoptosis. Its expression is dynamically regulated by mechanical stretch, hypoxia, and pathological stimuli like cardiac hypertrophy or heart failure, positioning it as a biomarker for cardiovascular diseases.
Recombinant ANKRD1 protein is engineered to study its molecular mechanisms and therapeutic potential. Typically produced in *E. coli* or mammalian expression systems, the purified protein retains functional domains, including the N-terminal ankyrin repeats and the C-terminal nuclear localization signal. Researchers utilize it to investigate its dual roles in cardioprotection and disease progression. For example, ANKRD1 exhibits anti-apoptotic effects under ischemic stress but may promote fibrosis in chronic overload. It also shows context-dependent roles in cancer, either suppressing tumors or enhancing metastasis. Recombinant ANKRD1 aids in identifying binding partners, validating signaling pathways (e.g., MAPK, NF-κB), and screening drug candidates targeting cardiac remodeling or muscular dystrophies. Its study bridges molecular insights with clinical applications, though challenges remain in understanding tissue-specific post-translational modifications and isoform diversity.
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