| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LZIC |
| Uniprot No | Q8WZA0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMASRGKT ETSKLKQNLE EQLDRLMQQL QDLEECREEL DTDEYEETKK ETLEQLSEFN DSLKKIMSGN MTLVDELSGM QLAIQAAISQ AFKTPEVIRL FAKKQPGQLR TRLAEMDRDL MVGKLERDLY TQQKVEILTA LRKLGEKLTA DDEAFLSANA GAILSQFEKV STDLGSGDKI LALASFEVEK TKK |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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LZIC (Leucine Zipper and ICAT Domain-Containing Protein) is a recombinant protein engineered to study cellular processes linked to protein-protein interactions and signaling pathways. The name derives from its two critical structural motifs: a leucine zipper, which facilitates dimerization with partner proteins, and an ICAT (Inhibitor of β-Catenin and TCF) domain, known for modulating Wnt/β-catenin signaling. This pathway is central to embryogenesis, tissue homeostasis, and cancer progression, making LZIC a subject of interest in developmental biology and oncology research.
Recombinant LZIC is typically produced using bacterial or mammalian expression systems, enabling high purity and scalability. Its design often includes affinity tags (e.g., His-tag) for simplified purification and detection. Researchers utilize LZIC to investigate its dual regulatory roles: the leucine zipper mediates interactions with transcription factors or kinases, while the ICAT domain competitively inhibits β-catenin/TCF complexes, potentially suppressing oncogenic Wnt signaling. Structural studies suggest conformational flexibility, allowing context-dependent functions in different cellular environments.
LZIC has been implicated in DNA damage response, cell cycle regulation, and apoptosis. Dysregulation of LZIC expression correlates with tumor aggressiveness in certain cancers, positioning it as a biomarker candidate or therapeutic target. Additionally, its role in balancing Wnt activity highlights relevance in regenerative medicine and stem cell research. Current challenges include elucidating post-translational modifications and tissue-specific interaction networks. As a recombinant tool, LZIC aids in deciphering mechanistic cross-talk between signaling cascades, offering insights for targeted drug development.
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