| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8;;S100A8 |
| WB Predicted band size | 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human S100A8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NOX3抗体的模拟参考文献(注：以下内容为示例，非真实文献)：
1. **文献名称**: *"NOX3-derived reactive oxygen species promote inner ear hair cell degeneration"*
**作者**: Smith A, et al.
**摘要**: 研究利用特异性NOX3抗体，发现NOX3在内耳毛细胞中高表达，其产生的ROS(活性氧)与年龄相关性听力损伤相关，抗体阻断实验表明抑制NOX3可减轻氧化损伤。
2. **文献名称**: *"Role of NOX3 in renal oxidative stress and fibrosis"*
**作者**: Chen L, et al.
**摘要**: 通过免疫组化和Western blot分析，研究者使用NOX3抗体证实其在慢性肾病模型肾脏组织中的表达上调，并发现NOX3通过激活TGF-β信号通路促进纤维化。
3. **文献名称**: *"NOX3 antibody-based detection of NADPH oxidase activity in cancer cells"*
**作者**: Kumar R, et al.
**摘要**: 该研究开发了一种高特异性NOX3单克隆抗体，用于检测多种癌细胞系中NOX3的活性，发现其与肿瘤侵袭性和化疗耐药性正相关。
4. **文献名称**: *"Targeting NOX3 in cardiovascular diseases: Insights from antibody inhibition studies"*
**作者**: García-Sánchez A, et al.
**摘要**: 利用NOX3中和抗体，研究显示其在高血压模型中可减少血管内皮氧化应激，改善血管功能，提示NOX3是心血管疾病的潜在治疗靶点。
(注意：以上文献及作者为虚构示例，实际研究需通过学术数据库检索。)
NOX3 (NADPH oxidase 3) is a member of the NADPH oxidase family, which plays a critical role in generating reactive oxygen species (ROS) by transferring electrons across biological membranes. Discovered in 2001. NOX3 is primarily expressed in inner ear tissues, particularly the vestibular and cochlear systems, where it contributes to physiological processes such as otoconia formation and hearing function. Unlike other NOX isoforms (e.g., NOX1. NOX2. NOX4), NOX3 exhibits restricted tissue distribution and requires specific regulatory subunits (e.g., NOXO1. NOXA1) for activation. Its dysregulation has been implicated in hearing loss, oxidative stress-related disorders, and neurodegenerative conditions.
NOX3 antibodies are essential tools for studying its expression, localization, and functional roles. These antibodies target specific epitopes within NOX3's structural domains, such as its transmembrane or intracellular regions. Researchers utilize them in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate NOX3's involvement in pathologies like noise-induced hearing loss, age-related hearing decline, and cisplatin-induced ototoxicity. Additionally, NOX3 antibodies aid in exploring its interaction with signaling pathways, including those linked to inflammation and apoptosis. Recent studies also highlight NOX3's potential role in cancer progression, particularly in tumors with oxidative stress-driven mechanisms. The development of selective NOX3 antibodies remains challenging due to structural similarities among NADPH oxidase isoforms, emphasizing the need for rigorous validation to ensure specificity in experimental models.
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