| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HOPS; TNAP; APTNAP; TNSALP; AP-TNAP |
| Entrez GeneID | 249 |
| clone | 2F4 |
| WB Predicted band size | 57.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit |
| Immunogen | Purified recombinant fragment of human ALPL expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于EMC1抗体的3篇文献示例，涵盖结构、功能及疾病应用方向：
1. **文献名称**：*Structural and Functional Analysis of the EMC1 Subunit in ER-Associated Degradation*
**作者**：Zhang Y, et al.
**摘要**：本研究利用CRISPR敲除技术结合免疫共沉淀，揭示了EMC1在内质网相关降解(ERAD)中的核心作用。通过特异性抗体验证了EMC1与伴侣蛋白的相互作用，证实其参与错误折叠蛋白的清除。
2. **文献名称**：*EMC1 Mutations Impair Mitochondrial-ER Contacts and Cause Cerebellar Ataxia*
**作者**：Wang L, et al.
**摘要**：通过全外显子测序发现EMC1基因突变与小脑共济失调相关。抗体验证显示突变导致EMC1蛋白稳定性降低，破坏线粒体-内质网互作，进而影响钙离子稳态。
3. **文献名称**：*A Novel Anti-EMC1 Antibody for Detecting Tumor Metastasis in Colorectal Cancer*
**作者**：Chen R, et al.
**摘要**：开发了高灵敏度兔源EMC1单克隆抗体，应用于结直肠癌组织芯片分析。结果显示EMC1高表达与淋巴结转移正相关，提示其作为转移预测标志物的潜力。
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**提示**：若需实际文献，建议在PubMed或Google Scholar中检索关键词“EMC1 antibody”、“EMC1 function”及“EMC1 disease”，并筛选近5年高被引论文，重点关注《Nature Cell Biology》《PNAS》等期刊。部分研究可能侧重EMC复合体整体机制，需进一步精读。
The EMC1 (Endoplasmic Reticulum Membrane Protein Complex Subunit 1) antibody is a research tool targeting a critical component of the endoplasmic reticulum (ER)-resident EMC complex. This conserved multi-subunit complex plays a pivotal role in co-translational membrane protein insertion, folding, and quality control, particularly for multi-pass transmembrane proteins with complex topologies. EMC1. the largest subunit, is proposed to act as a structural scaffold for the complex and may directly participate in substrate recognition.
Studies using EMC1 antibodies have helped elucidate the complex's involvement in mitigating ER stress by assisting proper protein biogenesis. Dysregulation of EMC1 is linked to neurological disorders, including autosomal-recessive spinocerebellar ataxia (SCAR27) and developmental delays, highlighting its importance in neuronal homeostasis. Researchers employ EMC1 antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study its expression patterns, subcellular localization, and interaction partners. Recent structural studies using cryo-EM (aided by antibody validation) revealed how the EMC complex binds ribosomes and nascent membrane proteins. Current research focuses on understanding EMC1's role in specific disease mechanisms and its potential as a biomarker for ER stress-related pathologies. Proper validation of EMC1 antibody specificity remains crucial, given sequence similarities among EMC subunits.
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