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A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CEA antibody.
Six vials standard (1 ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial HRP-conjugated CEA antibody(6 ml/bottle) --- Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.
One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.
One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.
One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
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