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Human carcinoembryonic antigen,CEA ELISA Kit

  • 中文名: 人癌胚抗原(CEA/CD66)ELISA Kit
货号: IPD-E04767h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人癌胚抗原(CEA/CD66)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CEACAM5含量。CEACAM5,也称为CEA或CD66e,是癌胚抗原相关细胞粘附分子家族的一员,主要在结直肠癌等上皮肿瘤细胞中高表达。其研究机制涉及细胞粘附、信号转导和肿瘤转移等过程,成为癌症免疫疗法的潜在靶点。试剂盒检测范围为5 ng/ml-80 ng/ml,灵敏度为2 ng/mL。本试剂盒适用于体外研究场景,例如肿瘤细胞分泌功能分析、癌症动物模型疗效评估、药物干预下CEA表达动态监测等科研领域,为探究CEA在肿瘤微环境中的作用机制及潜在治疗靶点研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Carcinoembryonic antigen ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; CD66e ELISA Kit; CEA ELISA Kit; Ceacam5 ELISA Kit; CEAM5_HUMAN ELISA Kit; DKFZp781M2392 ELISA Kit; Meconium antigen 100 ELISA Kit; OTTHUMP00000199032 ELISA Kit; OTTHUMP00000199033 ELISA Kit; OTTHUMP00000199034 ELISA Kit
  • 缩写:
    CEACAM5
  • Uniprot No.:
    P06731
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    5 ng/ml-80 ng/ml
  • 灵敏度:
    2 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cancer
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
  • 线性度:
  • 回收率:
  • 标准曲线:
  • 本试剂盒所含材料:



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CEA antibody.

    • Six vials standard (1 ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial HRP-conjugated CEA antibody(6 ml/bottle) --- Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.

    • One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.

    • One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.

    • One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.

    • 本试剂盒不含材料:



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期:
        3-5 working days

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