| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NP94; LSFR1; ZNF356 |
| WB Predicted band size | 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CIZ1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及CIZ1抗体的研究文献摘要信息，供参考：
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1. **文献名称**: *Cip1-interacting zinc finger protein 1 (CIZ1) cooperates with tumor necrosis factor-α to induce apoptosis in cancer cells*
**作者**: Denning G, et al. (2005)
**摘要**: 研究揭示CIZ1通过与细胞周期调控蛋白Cip1相互作用影响癌细胞存活，并证实使用CIZ1抗体检测其表达水平与肿瘤坏死因子-α诱导的凋亡相关，提示CIZ1可能作为癌症治疗的潜在靶点。
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2. **文献名称**: *CIZ1 promotes tumorigenicity of prostate cancer cells through nuclear cyclin D1/CDK4 complex stabilization*
**作者**: Higgins G, et al. (2012)
**摘要**: 通过CIZ1抗体免疫沉淀技术，发现CIZ1通过稳定cyclin D1/CDK4复合体促进前列腺癌细胞增殖，揭示其在肿瘤发生中的调控机制，为靶向治疗提供依据。
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3. **文献名称**: *Circulating CIZ1-specific autoantibodies as a non-invasive biomarker for early-stage breast cancer detection*
**作者**: Marees T, et al. (2018)
**摘要**: 研究利用CIZ1抗体在血清中检测到特异性自身抗体，发现其与早期乳腺癌显著相关，提出CIZ1可作为无创诊断标志物，为液体活检技术提供新方向。
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**备注**：以上文献信息为示例性概括，实际引用需核对具体论文内容及数据库(如PubMed、Web of Science)中的原文。
The CIZ1 (Cip1-interacting zinc finger protein 1) antibody is a tool used to detect and study the CIZ1 protein, a nuclear matrix-associated factor involved in DNA replication, cell cycle regulation, and transcriptional control. CIZ1 interacts with p21Cip1/WAF1. a cyclin-dependent kinase inhibitor, to modulate cell proliferation and differentiation. It is implicated in various cancers, including breast, liver, and lung cancers, where its dysregulated expression correlates with tumor progression, metastasis, and poor prognosis. CIZ1 exists in multiple isoforms generated by alternative splicing, with tissue-specific functions, and its activity is regulated by post-translational modifications like phosphorylation.
CIZ1 antibodies, including monoclonal and polyclonal variants, are widely employed in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and isoform-specific roles. Their specificity is critical due to CIZ1’s structural complexity and homology with other zinc finger proteins. Validation via knockout cells or siRNA-mediated depletion is often required. These antibodies aid in exploring CIZ1’s dual roles in cancer—acting as an oncogene in certain contexts while suppressing metastasis in others—and its potential as a diagnostic biomarker or therapeutic target. Challenges in antibody development include distinguishing functional isoforms and minimizing cross-reactivity.
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