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Human complement factor H,CFH ELISA Kit

  • 中文名： 人补体因子H(CFH)ELISA Kit
货号: IPD-E08931h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人补体因子H(CFH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的CFH含量。CFH即补体因子H，是补体系统重要调节蛋白。其背景上，在维持补体激活和抑制平衡中起关键作用。研究机制方面，主要是通过与补体C3b结合，抑制补体旁路途径过度激活，异常时可引发多种疾病，如年龄相关性黄斑变性等。试剂盒检测范围为31.2 ng/ml- 2000 ng/ml，灵敏度为7.8 ng/ml。适用于基础科研领域，如研究补体系统在自身免疫性疾病、肾脏病理或炎症反应中的作用，或用于评估疾病模型动物或体外实验中CFH的表达水平变化。其宽泛的检测范围可满足不同浓度样本的检测需求，支持多样本类型兼容性，为探索补体相关分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    adrenomedullin binding protein ELISA Kit; age related maculopathy susceptibility 1 ELISA Kit; AHUS 1 ELISA Kit; AHUS1 ELISA Kit; AMBP 1 ELISA Kit; AMBP1 ELISA Kit; ARMD 4 ELISA Kit; ARMD4 ELISA Kit; ARMS 1 ELISA Kit; ARMS1 ELISA Kit; beta 1 H globulin ELISA Kit; beta 1H ELISA Kit; beta1H ELISA Kit; CFAH_HUMAN ELISA Kit; CFH ELISA Kit; CFHL 3 ELISA Kit; CFHL3 ELISA Kit; Complement factor H ELISA Kit; complement factor H; isoform b ELISA Kit; Factor H ELISA Kit; factor H like 1 ELISA Kit; FH ELISA Kit; FHL 1 ELISA Kit; FHL1 ELISA Kit; H factor 1 (complement) ELISA Kit; H factor 1 ELISA Kit; H factor 2 (complement) ELISA Kit; HF 1 ELISA Kit; HF 2 ELISA Kit; HF ELISA Kit; HF1 ELISA Kit; HF2 ELISA Kit; HUS ELISA Kit; MGC88246 ELISA Kit
  • 缩写：
    CFH
  • Uniprot No.：
    P08603
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.2 ng/mL-2000 ng/mL
  • 灵敏度：
    7.8 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)245.723250.139256.612242.927251.751258.647SD0.0310.0420.0510.0420.0540.060CV(%)3.7134.9655.9165.0726.3536.920
  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CFH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:200

    Average %

    89


    Range %

    80-94


    1:400

    Average %

    97


    Range %

    91-102


    1:800

    Average %

    101


    Range %

    94-107


    1:1600

    Average %

    93


    Range %

    86-99









  • 回收率：

    The recovery of human CFH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-99


    EDTA plasma (n=4)

    102

    96-105









  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







































































    ng/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.566

    2.466

    2.516

    2.424


    1000

    2.031

    1.931

    1.981

    1.889


    500

    1.409

    1.298

    1.354

    1.262


    250

    0.867

    0.812

    0.840

    0.748


    125

    0.524

    0.514

    0.519

    0.427


    62.5

    0.345

    0.335

    0.340

    0.248


    31.2

    0.213

    0.203

    0.208

    0.116


    0

    0.094

    0.090

    0.092











  • 货期：
    3-5 working days

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