| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NCLX; NCKX6; SLC24A6 |
| WB Predicted band size | 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human SLC8B1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SLC8B1(NCLX)抗体的代表性文献(示例为虚构，供参考格式)：
1. **文献名称**: *Mitochondrial Na+/Ca2+ exchanger isoform 1 (NCLX) regulates cardiac mitochondrial calcium handling*
**作者**: Palty R, et al.
**摘要**: 本研究首次鉴定了线粒体钠钙交换蛋白NCLX(SLC8B1)的分子特性，并通过特异性抗体验证其在线粒体膜上的定位，证明其在心肌细胞钙外排中的关键作用。
2. **文献名称**: *NCLX antibody-based detection of mitochondrial calcium dynamics in neuronal cells*
**作者**: Luongo TS, et al.
**摘要**: 开发并验证了一种高特异性SLC8B1抗体，用于免疫印迹和免疫荧光实验，揭示了NCLX在神经元线粒体钙稳态中的功能及其与神经退行性疾病的相关性。
3. **文献名称**: *SLC8B1 knockout mice exhibit impaired mitochondrial calcium efflux and cardiomyopathy*
**作者**: Kostic M, et al.
**摘要**: 通过SLC8B1抗体检测基因敲除模型中的蛋白表达缺失，证实NCLX缺失导致心肌线粒体钙超载和心功能异常，为心脏病机制研究提供依据。
4. **文献名称**: *Commercial SLC8B1 antibodies: Validation and application in cancer cell studies*
**作者**: Domingo G, et al.
**摘要**: 系统性评估了市售SLC8B1抗体的特异性及适用性，并应用于多种癌细胞系，揭示NCLX在肿瘤代谢重编程中的潜在作用。
(注：上述文献为示例，实际引用需根据真实发表论文调整。)
The solute carrier family 8 member B1 (SLC8B1), also known as the mitochondrial sodium-calcium exchanger (NCLX), is a critical transporter protein localized to the inner mitochondrial membrane. It facilitates the exchange of sodium (Na⁺) and calcium (Ca²⁺) ions across the mitochondrial membrane, playing a pivotal role in regulating mitochondrial Ca²⁺ homeostasis. This process is essential for maintaining cellular energy production, calcium signaling, and apoptosis regulation. Dysregulation of SLC8B1/NCLX has been implicated in various pathological conditions, including neurodegenerative diseases (e.g., Parkinson’s disease), cardiac disorders (e.g., ischemia-reperfusion injury), and cancer.
SLC8B1 antibodies are immunological tools designed to detect and quantify the expression, localization, and functional dynamics of the NCLX protein in research settings. These antibodies are widely utilized in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to study mitochondrial calcium handling in both physiological and disease contexts. Their specificity and reliability are critical for elucidating the molecular mechanisms linking mitochondrial Ca²⁺ dysregulation to cellular dysfunction. Recent studies have highlighted SLC8B1/NCLX as a potential therapeutic target, driving the development and validation of high-affinity antibodies for preclinical investigations. Researchers prioritize antibodies with cross-reactivity validated across species (e.g., human, mouse) to ensure translational relevance in disease models.
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