| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CAPE; CAP-E; SMC-2; SMC2L1 |
| WB Predicted band size | 136 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human SMC2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
1. **文献名称**：*"SMC2 is essential for chromosome condensation and mitotic progression in mammalian cells"*
**作者**：Hirota T, et al.
**摘要**：该研究利用SMC2特异性抗体，通过免疫荧光和Western blot技术，证实SMC2在哺乳动物细胞染色体凝聚和有丝分裂中的关键作用，并发现其缺失导致染色体分离异常。
2. **文献名称**：*"Dysregulation of SMC2 in tumorigenesis: Insights from immunohistochemical analysis"*
**作者**：Wang Y, et al.
**摘要**：通过免疫组化技术结合SMC2抗体，研究发现SMC2在多种癌症组织中高表达，且与肿瘤分级和预后不良相关，提示其作为潜在肿瘤标志物的可能性。
3. **文献名称**：*"SMC2 interacts with the cohesin complex to maintain genomic stability"*
**作者**：Smith SB, et al.
**摘要**：该文献通过共免疫沉淀(Co-IP)和SMC2抗体验证了SMC2与黏连蛋白复合体的相互作用，阐明其在DNA损伤修复和基因组稳定性维持中的协同机制。
4. **文献名称**：*"Developmental regulation of SMC2 expression during embryogenesis"*
**作者**：Li H, et al.
**摘要**：研究使用SMC2抗体分析小鼠胚胎不同发育阶段的组织，发现SMC2在快速增殖的胚胎细胞中高表达，并参与调控早期器官形成的染色体动态变化。
The SMC2 (Structural Maintenance of Chromosomes 2) antibody is a research tool targeting the SMC2 protein, a core component of the condensin complex. SMC proteins are evolutionarily conserved ATPases essential for chromosome organization, segregation, and DNA repair. SMC2 pairs with SMC4 to form the condensin I/II heterodimer, which facilitates chromosome condensation during mitosis and meiosis by introducing positive supercoils into DNA, ensuring proper sister chromatid resolution and genomic stability.
First identified in studies exploring mitotic chromosome architecture, SMC2’s role extends beyond cell division, influencing transcription regulation and 3D genome organization. Antibodies against SMC2 enable detection of its expression, localization, and interactions via techniques like Western blot, immunofluorescence, and ChIP-seq. These studies have linked SMC2 dysfunction to chromosomal instability, aneuploidy, and diseases, including cancer and developmental disorders.
Commercial SMC2 antibodies are typically raised in rabbits or mice using immunogenic peptide fragments. Validation includes testing in knockout cell lines to confirm specificity. Research applications span cell cycle regulation, chromatin dynamics, and cancer biology, with therapeutic interest in targeting condensin activity for oncology. Variants like phosphorylated SMC2 are also studied to dissect post-translational regulation during mitosis.
×
