| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | LIN41; LIN-41 |
| WB Predicted band size | 93 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human TRIM71 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TRIM71抗体的3篇代表性文献摘要整理：
1. **文献名称**：*TRIM71 Cooperates with microRNAs to Repress Cdkn1a and Promote Embryonic Stem Cell Proliferation*
**作者**：Chen Y. et al.
**摘要**：研究利用TRIM71抗体验证其在胚胎干细胞中的蛋白表达，发现TRIM71通过抑制细胞周期抑制因子Cdkn1a促进干细胞增殖，依赖其RNA结合能力与miRNA协同作用。
2. **文献名称**：*TRIM71 as a Novel Diagnostic Marker in Hepatocellular Carcinoma*
**作者**：Wang L. et al.
**摘要**：通过免疫组化(使用TRIM71特异性抗体)发现，TRIM71在肝癌组织中显著高表达，且与患者生存率负相关，提示其可作为潜在治疗靶点。
3. **文献名称**：*Development of a High-Affinity Monoclonal Antibody for TRIM71 Functional Studies*
**作者**：Zhang R. et al.
**摘要**：报道了一种新型TRIM71单克隆抗体的开发与验证，该抗体成功用于免疫共沉淀(Co-IP)和活细胞成像，揭示了TRIM71在细胞质中的动态定位。
(注：以上文献信息为模拟示例，实际引用请通过学术数据库检索确认。)
TRIM71. a member of the tripartite motif (TRIM) protein family, is an RNA-binding E3 ubiquitin ligase implicated in post-transcriptional gene regulation and embryonic development. Structurally, it contains a RING finger domain (mediating ubiquitination), B-box domains, and a coiled-coil region, along with a C-terminal NHL domain crucial for RNA interaction. TRIM71 plays a key role in stem cell maintenance, cellular reprogramming, and tissue regeneration by regulating microRNA (e.g., let-7) activity and mRNA stability. It is essential during early embryogenesis, with knockout studies in mice showing lethal developmental defects.
In disease contexts, TRIM71 is dysregulated in cancers (e.g., hepatocellular carcinoma, glioblastoma) and linked to tumorigenesis through pathways like Wnt/β-catenin and MYC. Its dual role as oncogene or tumor suppressor depends on cellular context. TRIM71 antibodies are critical tools for detecting protein expression, localization, and interaction partners in studies of development, pluripotency, and cancer. They are validated for techniques including Western blotting, immunofluorescence, and immunoprecipitation. High-quality antibodies require specificity testing via knockout controls due to potential cross-reactivity with other TRIM proteins. Research applications span basic mechanisms of RNA-protein interactions to translational studies exploring TRIM71 as a therapeutic target.
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