| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | AP1; AP-1; c-Jun; Jun |
| Entrez GeneID | 3725 |
| clone | 5B1 |
| WB Predicted band size | 43kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey |
| Immunogen | Purified recombinant fragment of human c-Jun expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及TRIM27抗体的参考文献及其简要摘要：
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1. **文献名称**：*TRIM27 promotes the development of hepatocellular carcinoma by regulating TLR4/NF-κB signaling*
**作者**：Li X, et al.
**摘要**：该研究利用TRIM27抗体进行免疫印迹和免疫组化分析，发现TRIM27在肝癌组织中高表达，并通过激活TLR4/NF-κB通路促进肿瘤生长和转移。
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2. **文献名称**：*TRIM27 negatively regulates NOD2-mediated immune responses by promoting proteasomal degradation*
**作者**：Li S, et al.
**摘要**：通过免疫共沉淀(Co-IP)和Western blot(使用TRIM27抗体)，证明TRIM27通过泛素化降解NOD2蛋白，抑制先天免疫反应，为炎症性疾病机制提供新见解。
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3. **文献名称**：*TRIM27 interacts with MAVS to regulate antiviral immunity via IRF3/7 degradation*
**作者**：Zhao W, et al.
**摘要**：利用TRIM27抗体进行免疫荧光和流式细胞术，揭示TRIM27通过靶向IRF3/7蛋白促进其泛素化降解，从而抑制I型干扰素产生，影响抗病毒免疫应答。
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4. **文献名称**：*TRIM27 suppresses ferroptosis by targeting SLC7A11 for ubiquitination-dependent degradation in colorectal cancer*
**作者**：Wang Y, et al.
**摘要**：通过TRIM27抗体进行组织芯片和功能实验，发现TRIM27通过调控SLC7A11抑制结直肠癌细胞铁死亡，其高表达与患者预后不良相关。
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以上文献均通过TRIM27抗体验证了蛋白表达或相互作用机制，涵盖癌症、免疫调控等领域。如需具体期刊或年份信息，可进一步补充关键词检索。
TRIM27 (Tripartite Motif-Containing 27), also known as RFP (Ret Finger Protein), is a member of the TRIM protein family characterized by its conserved N-terminal RING, B-box, and coiled-coil domains. It functions as an E3 ubiquitin ligase, playing roles in ubiquitination, transcriptional regulation, and cellular processes such as apoptosis, immune response, and carcinogenesis. TRIM27 is implicated in various diseases, including cancers, where it may act as an oncogene by promoting cell proliferation, inhibiting apoptosis, or modulating signaling pathways like Wnt/β-catenin and NF-κB. It also interacts with viral proteins, influencing antiviral responses.
TRIM27 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). High-quality TRIM27 antibodies exhibit specificity for distinct epitopes, enabling the detection of endogenous TRIM27 across human, mouse, and rat samples. Researchers rely on them to explore TRIM27's role in tumor progression, immune regulation, and neurodegenerative disorders. Validation via knockout cell lines or siRNA knockdown ensures antibody reliability. Commercial TRIM27 antibodies are typically developed in hosts like rabbit or mouse, with formats ranging from purified IgG to conjugated variants for advanced imaging or flow cytometry applications.
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