| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | MgcRabGAP |
| WB Predicted band size | 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human TBC1D21 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TBC1D21抗体的3篇参考文献示例(文献信息为模拟创作，仅供参考)：
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1. **文献名称**: *TBC1D21 regulates ciliogenesis through interaction with Rab GTPases*
**作者**: Zhang Y, et al.
**摘要**: 本研究利用TBC1D21抗体进行免疫荧光和共聚焦显微成像，发现TBC1D21通过其TBC结构域与Rab8A和Rab11相互作用，调控纤毛形成过程。抗体特异性验证显示其在敲低细胞系中信号显著降低。
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2. **文献名称**: *Role of TBC1D21 in intracellular vesicle trafficking*
**作者**: Lee S, et al.
**摘要**: 通过Western blot和免疫沉淀实验，作者发现TBC1D21抗体可特异性识别HeLa细胞中的内源性蛋白。研究揭示TBC1D21参与内体-高尔基体运输，影响EGFR信号通路的循环过程。
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3. **文献名称**: *TBC1D21 as a novel binding partner of mTORC1 signaling complex*
**作者**: Gupta R, et al.
**摘要**: 该研究使用TBC1D21抗体进行蛋白质互作分析，发现其与mTORC1复合物亚基存在共定位。功能实验表明TBC1D21可能通过调控mTORC1活性影响细胞代谢与自噬。
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**注意**：上述文献为示例，实际引用需查询PubMed/Google Scholar等平台。建议检索关键词“TBC1D21 antibody”、“TBC1D21 function”获取真实文献。
TBC1D21 (Tre-2/Bub2/Cdc16 domain family member 21) is a protein encoded by the TBC1D21 gene, which belongs to the TBC/RABGAP family known for regulating Rab GTPases involved in intracellular trafficking and membrane dynamics. TBC1D21 contains a conserved TBC domain that facilitates GTPase-activating protein (GAP) activity, potentially modulating Rab-mediated processes such as vesicle transport, organelle organization, or ciliary function. Studies suggest its association with primary cilia, specialized sensory organelles critical for signaling pathways (e.g., Hedgehog) and cellular homeostasis.
Antibodies targeting TBC1D21 are essential tools for investigating its expression, localization, and functional roles. These antibodies are typically produced using immunogens derived from specific regions of the human TBC1D21 protein, such as recombinant peptides or epitope-tagged constructs. Validated applications include Western blotting, immunofluorescence, and immunoprecipitation, enabling researchers to study tissue-specific expression patterns, subcellular distribution (e.g., ciliary base or centrosomal regions), and interactions with trafficking-related proteins. Proper antibody validation via knockout controls or siRNA-mediated depletion is crucial due to potential cross-reactivity with homologous TBC family members. TBC1D21 antibodies have been utilized in research exploring ciliopathies, neurodevelopmental disorders, and intracellular transport mechanisms, though its precise physiological and pathological roles remain under active investigation. Commercial availability and species reactivity (e.g., human, mouse) vary by supplier.
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