| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | TAF2A; TAF2H; TAFII30 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human TAF10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于TAF10抗体的参考文献示例(文献信息为模拟生成，实际引用需核实原文)：
1. **名称**：*TAF10 is required for the integrity of the TFIID complex and embryonic stem cell pluripotency*
**作者**：M. Talamillo et al.
**摘要**：研究通过TAF10特异性抗体敲除实验，发现TAF10对维持TFIID复合体稳定性及胚胎干细胞多能性至关重要，抗体用于ChIP-seq证实TAF10在核心启动子区域的富集。
2. **名称**：*Differential roles of TAF10 in transcriptional regulation by nuclear receptors*
**作者**：J. Frontini et al.
**摘要**：利用TAF10抗体进行免疫共沉淀和Western blot，揭示TAF10在核受体(如PPARγ)介导的转录激活中具有受体特异性功能，影响靶基因表达。
3. **名称**：*TAF10 interacts with the GCN5 acetyltransferase to coordinate histone modifications and gene expression*
**作者**：S. Lee et al.
**摘要**：通过TAF10抗体进行蛋白质互作分析，证明TAF10与组蛋白乙酰转移酶GCN5形成复合物，协同调控组蛋白H3K9乙酰化及下游基因转录。
4. **名称**：*Loss of TAF10 in hepatocytes disrupts lipid metabolism via altered chromatin accessibility*
**作者**：K. Müller et al.
**摘要**：研究使用TAF10抗体进行肝脏特异性敲除模型分析，发现TAF10缺失通过改变染色质开放性导致脂代谢紊乱，抗体用于验证TAF10蛋白表达水平。
(注：以上文献为示例，实际引用需根据具体研究检索PubMed等数据库获取。)
The TAF10 antibody is a crucial tool in studying the role of TATA-box binding protein-associated factor 10 (TAF10), a subunit of the TFIID complex, which is essential for RNA polymerase II-mediated transcription. TAF10. part of the transcription factor IID (TFIID), binds TBP (TATA-box binding protein) and other TAFs to form a multiprotein complex that recognizes core promoters, facilitating the assembly of the preinitiation complex. TAF10 plays a dual role in transcription regulation, contributing to both basal and activated transcription by interacting with activators, chromatin modifiers, and other cofactors. Its involvement in histone acetylation and chromatin remodeling underscores its importance in maintaining transcriptional fidelity and cell viability.
Studies have linked TAF10 to critical biological processes, including cell cycle regulation, differentiation, and development. Dysregulation or mutations in TAF10 are associated with diseases such as cancer and neurodegenerative disorders, highlighting its biomedical relevance. The TAF10 antibody enables researchers to investigate its expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence. Specificity and sensitivity of the antibody are vital for distinguishing TAF10 from homologous proteins and validating its role in transcriptional mechanisms. By elucidating TAF10's functions, this antibody contributes to understanding gene regulation dynamics and potential therapeutic targets in disease contexts.
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