| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ATP5G1; ATP5G2; ATP5G3;;ATP5MC1 |
| WB Predicted band size | Calculated MW: 14 kDa ; Observed MW: 8 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ATP5MC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4条关于NUP98抗体的参考文献及其摘要内容：
1. **文献名称**：*"NUP98 Fusion in Hematologic Malignancies: Role in Leukemogenesis and Therapeutic Implications"*
**作者**：Takeshita A, et al.
**摘要**：探讨NUP98基因融合在急性髓系白血病(AML)中的致癌机制，通过抗体检测发现NUP98融合蛋白异常表达可激活HOXA基因，促进白血病干细胞增殖。
2. **文献名称**：*"Immunohistochemical Detection of NUP98-HOXA9 Fusion Proteins in Leukemia Patients"*
**作者**：Slape C, et al.
**摘要**：开发特异性抗体用于免疫组化检测NUP98-HOXA9融合蛋白，证实其在AML患者中的高表达，并提出其作为潜在诊断标志物的临床应用价值。
3. **文献名称**：*"Nuclear Pore Complex Protein NUP98 as a Biomarker in Myelodysplastic Syndromes"*
**作者**：Wang X, et al.
**摘要**：通过Western blot和免疫荧光技术，发现NUP98抗体检测的核定位异常与骨髓增生异常综合征(MDS)向白血病转化相关，提示其预后评估作用。
4. **文献名称**：*"Functional Characterization of NUP98 Antibodies in Model Organisms"*
**作者**：Griffis ER, et al.
**摘要**：利用斑马鱼和小鼠模型，验证多种NUP98抗体的特异性，揭示NUP98在胚胎发育和造血系统中的关键功能缺失表型。
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**注**：以上文献为示例，实际引用时需核对真实数据库(如PubMed)中的具体信息。
The NUP98 antibody is a crucial tool in studying the nucleoporin protein NUP98. a component of the nuclear pore complex (NPC) that regulates nucleocytoplasmic transport of RNA and proteins. NUP98 is encoded by the *NUP98* gene located on chromosome 11p15.5 and is involved in cell differentiation, gene regulation, and mitosis. It gained prominence due to its role in chromosomal translocations linked to hematologic malignancies, particularly acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). Over 30 fusion partners of NUP98 have been identified (e.g., *HOXA9*, *NSD1*), creating oncogenic chimeric proteins that disrupt transcriptional regulation and drive leukemogenesis.
NUP98 antibodies are used to detect wild-type NUP98 or its fusion variants in research and diagnostics. They help localize the protein within the nuclear membrane, assess expression levels, and identify aberrant fusion proteins in patient samples. These antibodies are vital for understanding the mechanistic role of NUP98 in normal cellular processes and disease, particularly in characterizing fusion-driven oncogenesis. Commercially available NUP98 antibodies are typically validated for techniques like Western blotting, immunofluorescence, and immunohistochemistry. Their specificity is critical, as NUP98 fusion proteins often retain epitopes from the N-terminal region of wild-type NUP98. Ongoing research leverages these antibodies to explore therapeutic targets and prognostic biomarkers in NUP98-rearranged cancers.
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