关键信息
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基因名
14-3-3 theta
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简介
14-3-3 theta proteins are adapters in multiple signaling pathways that recognize phosphoserine or phosphothreonine motifs and modulate chaperone activity upon binding. It negatively regulates PDPK1 kinase activity, forms homodimers, and interacts with CDK16, phosphorylated RGS7, SSH1, phosphorylated CDKN1B, GAB2, phosphorylated PDPK1, and phosphorylated DAPK2. 14-3-3 theta Protein, Human (GST) is the recombinant human-derived 14-3-3 theta protein, expressed by E. coli , with N-GST labeled tag.
- 应用
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别名
14-3-3 protein theta; 14-3-3 protein tau; Protein HS1; YWHAQ
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种属
Human
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表达系统
E. coli
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标签
N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P27348
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表达区间
M1-N245
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蛋白长度
Full Length
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分子量
53 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
14-3-3 theta is a member of the 14-3-3 protein family, which plays critical roles in various cellular processes, including cell growth, differentiation, and stress responses. Research into 14-3-3 theta has gained momentum due to its involvement in important signaling pathways, particularly those related to cancer progression and neurological disorders. These proteins function as molecular scaffolds, modulating the activity of numerous target proteins by binding to specific phosphoserine-containing motifs. The unique expression pattern of 14-3-3 theta in specific tissues and its differential regulation in pathological conditions underscore its potential as a biomarker and therapeutic target. Recent studies have indicated that alterations in the expression levels of 14-3-3 theta may influence cellular responses to stressors, suggesting its role in maintaining cellular homeostasis. Understanding the precise mechanisms by which 14-3-3 theta interacts with its partners could provide insights into its functional significance and contribute to the development of new strategies for treating diseases where these interactions are disrupted. This ongoing research aims to unravel the complex regulatory networks involving 14-3-3 theta, highlighting its significance in health and disease.
