| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KRML; MCTO |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human MAFB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAFB抗体的3篇参考文献示例(内容为虚构，仅供参考格式)：
1. **《MAFB expression in macrophage differentiation and function》**
- **作者**: Motoda L, et al.
- **摘要**: 研究通过MAFB特异性抗体分析其在巨噬细胞极化中的作用，发现MAFB高表达于M2型巨噬细胞，并调控IL-10等抗炎因子分泌，提示其在免疫调控中的关键地位。
2. **《MAFB as a diagnostic marker in multiple myeloma》**
- **作者**: Nishida K, et al.
- **摘要**: 利用MAFB抗体进行免疫组化及流式分析，证实MAFB在多发性骨髓瘤细胞中特异性高表达，可作为区分浆细胞疾病的新型生物标志物。
3. **《MAFB regulates epidermal barrier formation in skin development》**
- **作者**: Chihiro S, et al.
- **摘要**: 通过MAFB抗体染色发现，MAFB在皮肤角质形成细胞分化中调控紧密连接蛋白表达，缺失导致小鼠模型出现严重表皮屏障缺陷。
4. **《MAFB in rheumatoid arthritis synovial fibroblasts》**
- **作者**: Morita Y, et al.
- **摘要**: 研究采用MAFB抗体检测类风湿关节炎患者滑膜细胞，发现MAFB通过NF-κB通路促进炎症因子释放，为潜在治疗靶点。
(注：以上文献为示例，实际引用需查询真实数据库如PubMed。)
The MAFB antibody is a crucial tool in biomedical research, targeting the MAFB transcription factor, a member of the large Maf protein family. MAFB (v-maf musculoaponeurotic fibrosarcoma oncogene homolog B) contains a basic leucine zipper (bZIP) domain, enabling DNA binding and dimerization to regulate gene expression. It plays pivotal roles in cell differentiation, development, and homeostasis, particularly in macrophages, osteoclasts, pancreatic β-cells, and kidney podocytes.
MAFB is implicated in diseases such as multiple myeloma (where its overexpression drives oncogenesis), diabetes (linked to β-cell dysfunction), and renal disorders. Researchers use MAFB antibodies in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression, localization, and dynamics in tissues or cultured cells. These antibodies aid in studying MAFB's regulatory mechanisms in immune responses, bone remodeling, and metabolic pathways.
Commercial MAFB antibodies are typically validated for specificity across human, mouse, and rat samples. However, cross-reactivity with other Maf proteins (e.g., MAFA, c-MAF) requires careful validation via knockout controls. Recent studies also explore MAFB's role in cancer metastasis and autoimmune diseases, expanding its therapeutic relevance. Overall, MAFB antibodies remain indispensable for unraveling its biological functions and pathological contributions.
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