| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | B-50; PP46 |
| WB Predicted band size | 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GAP43 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于GAP43抗体的3篇代表性文献及其摘要内容：
---
1. **文献名称**：*Growth-associated protein GAP-43: a specific marker for nerve regeneration*
**作者**：Benowitz LI, Routtenberg A
**摘要**：该研究验证了GAP43抗体作为轴突再生标志物的特异性，发现其在周围神经损伤后再生神经元中高表达，而在未受损神经元中表达较低，提示GAP43抗体可用于追踪神经再生过程。
---
2. **文献名称**：*A protein induced during nerve growth (GAP-43) is a major component of growth-cone membranes*
**作者**：Skene JH, Jacobson RD
**摘要**：通过GAP43抗体标记生长锥，证明GAP43在神经元发育和突触可塑性中的关键作用，揭示了其在神经生长导向和突触重塑中的功能。
---
3. **文献名称**：*B-50/GAP-43 in neuronal development and repair*
**作者**：Oestreicher AB, De Graan PN
**摘要**：综述GAP43抗体的应用，总结了其在研究神经元发育、损伤修复及学习记忆机制中的重要性，并讨论了其在不同物种中的保守性。
---
这些文献均围绕GAP43抗体的功能验证和应用展开，涵盖神经再生、发育及分子机制研究。
**Background of GAP43 Antibody**
The Growth Associated Protein 43 (GAP43), also known as neuromodulin or B-50. is a neuron-specific protein crucial for axonal growth, synaptic plasticity, and nerve regeneration. It is highly expressed during neuronal development, particularly in growing axons and growth cones, and its levels decline in most mature neurons. However, GAP43 re-emerges during nerve injury or regeneration, making it a key marker for studying neurodevelopmental processes and repair mechanisms.
GAP43 antibodies are widely used to detect this protein in research applications, including immunohistochemistry (IHC), Western blotting, and immunofluorescence (IF). These antibodies help investigate neural development, synaptic remodeling, and responses to neural injury. GAP43’s role in modulating intracellular signaling pathways, such as protein kinase C (PKC) activity, further links it to learning, memory, and neurodegenerative disorders like Alzheimer’s and Parkinson’s diseases.
Structurally, GAP43 is a 24 kDa protein with a conserved palmitoylation site for membrane anchoring and a PKC phosphorylation domain. Antibodies targeting specific epitopes (e.g., phosphorylated residues) enable functional studies of its post-translational modifications. Both monoclonal and polyclonal GAP43 antibodies are available, with validation often including knockout controls to ensure specificity.
Overall, GAP43 antibodies serve as essential tools in neuroscience, offering insights into neural repair, developmental biology, and disease mechanisms.
×
