| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | R4; T6; CD1; FCB6; HTA1; CD1A |
| Entrez GeneID | 909 |
| clone | 6H3 |
| WB Predicted band size | 37kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD1A expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CAPN14抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Genetic, epigenetic, and mechanistic insights into CAPN14-mediated eosinophilic esophagitis"*
**作者**：Rothenberg ME, et al.
**摘要**：该研究通过基因关联分析和功能实验，发现CAPN14在嗜酸性食管炎(EoE)患者的食管上皮中特异性高表达。研究使用抗CAPN14抗体进行免疫组化和Western blot验证，证实IL-13等细胞因子通过表观遗传调控增强CAPN14表达，导致食管屏障功能异常。
2. **文献名称**：*"CAPN14 expression in esophageal mucosa: A potential biomarker for eosinophilic esophagitis"*
**作者**：Davis BP, et al.
**摘要**：本研究评估CAPN14作为EoE诊断标志物的潜力，利用特异性抗CAPN14抗体对食管活检样本进行免疫染色。结果显示，CAPN14蛋白水平与疾病活动度显著相关，提示其可作为治疗靶点或临床分层的依据。
3. **文献名称**：*"Functional characterization of CAPN14 variants in allergic inflammation"*
**作者**：Kottyan LC, et al.
**摘要**：研究结合CRISPR/Cas9基因编辑和CAPN14抗体介导的蛋白检测，发现EoE相关CAPN14基因变异导致蛋白酶活性异常，促进上皮细胞炎症因子释放，揭示了其在Th2型免疫反应中的关键作用。
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**备注**：上述文献为示例，实际引用时需核对具体发表年份及期刊。若需全文或详细方法，建议通过PubMed或学术数据库检索标题或作者信息。
Calpain 14 (CAPN14) is a calcium-dependent cysteine protease belonging to the calpain family, which plays roles in cellular processes such as signal transduction, apoptosis, and cytoskeletal remodeling. CAPN14 gained attention due to its association with eosinophilic esophagitis (EoE), a chronic allergic inflammatory disorder. Genome-wide studies identified CAPN14 as a susceptibility gene highly expressed in the esophageal epithelium, particularly under interleukin-13 (IL-13) stimulation, a cytokine linked to EoE pathogenesis. CAPN14 is thought to contribute to epithelial barrier dysfunction and inflammatory responses in EoE by modulating protease activity and intracellular signaling pathways.
CAPN14 antibodies are essential tools for investigating its expression, localization, and function. These antibodies are typically developed using immunogenic peptides or recombinant protein fragments and validated for specificity in assays like Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF). Research-grade antibodies help elucidate CAPN14's role in EoE and its interaction with pathways involving calpain-mediated proteolysis, epithelial integrity, and immune activation. Some studies also explore CAPN14 as a potential therapeutic target, with inhibitors under investigation. However, challenges remain in understanding its precise substrates and regulatory mechanisms. CAPN14 antibodies thus serve as critical reagents for advancing mechanistic and translational studies in allergic diseases and calpain biology.
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