| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCM7 |
| Uniprot No | P33993-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389aa |
| 氨基酸序列 | MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYV DLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLD VYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSSKPRVIR EVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTF MPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIP RSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEA HRIVKMNKSEDDESGAGELTREELRQIADVIFATVRELVSGGRSVRFSEA EQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV |
| 预测分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MCM7 Recombinant Protein**
MCM7 (Minichromosome Maintenance Complex Component 7) is a critical subunit of the hexameric MCM2-7 complex, a core component of the eukaryotic DNA replication machinery. This complex plays an essential role in the initiation and elongation phases of DNA replication by functioning as a replicative helicase, unwinding double-stranded DNA to enable replication fork progression. MCM7. along with other MCM proteins, forms a ring-shaped structure that binds to chromatin during the G1 phase of the cell cycle, licensing replication origins for a single use per cycle to ensure genomic stability.
The recombinant MCM7 protein is engineered through molecular cloning techniques, typically expressed in systems like *E. coli*, yeast, or mammalian cells, followed by purification to near homogeneity. Recombinant expression allows for the production of large quantities of highly pure, biologically active MCM7. facilitating *in vitro* studies to dissect its structural and functional roles. Researchers utilize this protein to investigate helicase activity, ATPase function, and interactions with other replication factors (e.g., Cdc45. GINS) or regulatory proteins (e.g., cyclin-dependent kinases).
MCM7 is also of clinical interest due to its overexpression in various cancers, correlating with uncontrolled cell proliferation and poor prognosis. Recombinant MCM7 serves as a tool for developing diagnostic assays or therapeutic strategies targeting DNA replication vulnerabilities in cancer. Additionally, it aids in studying replication stress, checkpoint responses, and mechanisms underlying diseases linked to replication errors. Overall, MCM7 recombinant protein is a vital resource for advancing both basic and translational research in molecular biology and oncology.
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