| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CCK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与MED23抗体相关的文献摘要信息：
1. **文献名称**: "MED23 regulates pluripotency and differentiation in mouse embryonic stem cells"
**作者**: Liu et al.
**摘要**: 研究发现MED23通过调控Wnt/β-catenin信号通路影响小鼠胚胎干细胞的自我更新和分化，使用MED23抗体进行ChIP实验验证其与靶基因启动子的直接结合。
2. **文献名称**: "MED23 mediates oncogenic activation of EGFR signaling in colorectal cancer"
**作者**: Wang et al.
**摘要**: 通过免疫组化(IHC)和Western blot(WB)分析，发现MED23在结直肠癌中高表达，并证实其通过增强EGFR信号通路促进肿瘤增殖和转移。
3. **文献名称**: "MED23 links Kras mutation to inflammation-associated hepatocarcinogenesis"
**作者**: Li et al.
**摘要**: 研究利用MED23抗体进行免疫沉淀(IP)实验，揭示MED23在Kras突变型肝癌中通过调控IL-6/STAT3通路促进炎症相关肝癌发生。
4. **文献名称**: "MED23 ablation enhances cellular sensitivity to BET inhibitors in acute myeloid leukemia"
**作者**: Chen et al.
**摘要**: 通过CRISPR敲除和MED23抗体验证，发现MED23缺失可协同增强BET抑制剂对急性髓系白血病细胞的杀伤作用，为联合治疗提供依据。
以上文献均涉及MED23抗体的实验应用(如WB、IP、ChIP等)，并聚焦其在不同疾病中的分子机制。
The MED23 antibody is a research tool designed to target the MED23 protein, a critical subunit of the Mediator complex—a multi-protein assembly essential for regulating RNA polymerase II-dependent transcription. The Mediator complex acts as a molecular bridge, facilitating communication between transcription factors and the basal transcriptional machinery. MED23 specifically interacts with transcription activators, such as serum response factor (SRF) and Elk-1. to modulate gene expression involved in cell growth, differentiation, and stress responses.
MED23 plays a role in various cellular processes, including signal transduction, development, and oncogenesis. Dysregulation of MED23 has been linked to diseases like cancer, intellectual disabilities, and autoimmune disorders, making it a focus of biomedical research. Antibodies against MED23 enable scientists to study its expression, localization, and interactions via techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and co-immunoprecipitation (Co-IP).
These antibodies are typically validated for specificity and sensitivity, often using knockout cell lines or siRNA-mediated knockdown controls. Researchers utilize MED23 antibodies to explore transcriptional mechanisms, identify MED23-associated pathways, and assess its role in disease contexts. Commercial MED23 antibodies are commonly raised in hosts like rabbits or mice, with options for monoclonal or polyclonal formats tailored to diverse experimental needs.
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