| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MED27 |
| Uniprot No | Q6P2C8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMADVINVSVNLEAFSQAISAIQALRSS VSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLV GKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGL LNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMS IHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGK LDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLF QAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MED27重组蛋白的3篇参考文献示例(内容基于虚构文献，供参考)：
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1. **文献名称**: "Recombinant MED27 Expression and Structural Characterization in Transcriptional Regulation"
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组MED27蛋白，通过X射线晶体学解析其三维结构，揭示了其与中介体复合物其他亚基的关键结合位点，为理解其在RNA聚合酶II转录中的调控机制提供结构基础。
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2. **文献名称**: "MED27 Recombinant Protein Interacts with p53 in DNA Damage Response"
**作者**: Patel S, et al.
**摘要**: 利用重组MED27蛋白进行体外Pull-down实验，发现其直接与肿瘤抑制蛋白p53相互作用，并在DNA损伤条件下增强p53介导的靶基因转录激活，提示MED27可能在癌症发生中具有调控作用。
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3. **文献名称**: "Functional Analysis of MED27 in Neuronal Differentiation Using Recombinant Protein Rescue Assays"
**作者**: Kim H, et al.
**摘要**: 通过构建MED27缺陷型细胞系，并引入重组MED27蛋白进行功能回补实验，证实MED27通过调控神经营养因子相关基因(如BDNF)的表达，影响神经元分化过程。
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(注：若需真实文献，建议在PubMed或Google Scholar中检索关键词“MED27 recombinant protein”。)
MED27 (Mediator Complex Subunit 27) is a critical component of the Mediator complex, a multi-protein assembly that regulates RNA polymerase II-dependent transcription in eukaryotes. This evolutionarily conserved complex acts as a molecular bridge, integrating regulatory signals from transcription factors and chromatin-modifying enzymes to orchestrate gene expression. MED27. located on human chromosome 9q34.3. encodes a 231-amino acid protein that interacts with other Mediator subunits (e.g., MED6. MED30) to stabilize the complex's architecture and modulate its functional dynamics.
Structurally, MED27 contains conserved domains facilitating protein-protein interactions, particularly within the Mediator's "middle module," which influences transcriptional initiation and elongation. Studies link MED27 to neuronal development, lipid metabolism, and cell cycle regulation. Its dysfunction has been implicated in diseases ranging from cancers (e.g., colorectal, hepatocellular carcinoma) to neurodegenerative disorders, with mutations associated with autosomal recessive childhood-onset Parkinsonism.
Recombinant MED27 proteins are typically produced using bacterial (E. coli) or mammalian expression systems, often fused with tags like GST or His for purification and detection. These engineered proteins enable functional studies to dissect MED27's role in transcription regulation, its binding partners (e.g., ELK1. CREB transcription factors), and post-translational modifications. Researchers employ recombinant MED27 in pull-down assays, chromatin immunoprecipitation (ChIP), and in vitro transcription systems to investigate Mediator complex mechanics. Recent applications extend to drug discovery platforms targeting transcriptional dysregulation and gene therapy vector development.
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