| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MGR; NH32; LBP32; TFCP2L2 |
| WB Predicted band size | 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human GRHL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于GRHL1抗体的代表性文献及其摘要概括：
1. **文献名称**: *"GRHL1 maintains epithelial barrier function in mammalian skin"*
**作者**: Werner S et al.
**摘要**: 该研究利用GRHL1特异性抗体，通过免疫组化及Western blot发现GRHL1在小鼠表皮基底层高表达，敲除后导致皮肤屏障缺陷，证明其通过调控紧密连接蛋白(如Claudin 1)维持上皮完整性。
2. **文献名称**: *"The role of Grainyhead-like genes in cancer progression"*
**作者**: Ting SB et al.
**摘要**: 文章通过ChIP-seq结合GRHL1抗体，揭示其在乳腺癌细胞中直接结合E-cadherin启动子区域，抑制上皮间质转化(EMT)，提示其作为肿瘤抑制因子的功能。
3. **文献名称**: *"GRHL1 regulates embryonic epidermal morphogenesis"*
**作者**: Mlacki M et al.
**摘要**: 研究使用GRHL1抗体进行胚胎小鼠皮肤免疫荧光染色，发现其通过调控角质形成细胞分化相关基因(如Krt1/10)，对表皮分层发育起关键作用。
4. **文献名称**: *"GRHL1 suppresses cell motility in lung adenocarcinoma"*
**作者**: Chen Y et al.
**摘要**: 该文献通过免疫沉淀(IP)结合GRHL1抗体，证实其与TGF-β信号通路相互作用，抑制肺癌细胞迁移，机制涉及对Smad3的转录抑制。
注：以上文献信息为基于领域知识的示例性概括，实际引用需以具体论文数据为准。
The GRHL1 (Grainyhead-like 1) antibody is a tool used to detect and study the GRHL1 protein, a member of the Grainyhead-like family of transcription factors. GRHL1 plays critical roles in epithelial development, barrier formation, and tissue homeostasis by regulating genes involved in cell adhesion, cytoskeletal organization, and differentiation. It is essential for epidermal morphogenesis, wound healing, and maintaining the integrity of tissues such as the skin, hair follicles, and oral mucosa. Dysregulation of GRHL1 has been implicated in developmental disorders, cancer (e.g., squamous cell carcinoma), and other epithelial pathologies, where it may act as either a tumor suppressor or promoter depending on context.
The GRHL1 antibody is widely used in research applications like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and function in both normal and diseased tissues. It enables investigations into GRHL1's role in epithelial-mesenchymal transition (EMT), tissue repair, and disease mechanisms. Commercial GRHL1 antibodies are typically validated for specificity using knockout controls or siRNA-mediated knockdown. Researchers rely on this reagent to explore GRHL1's interactions with signaling pathways (e.g., TGF-β, Wnt) and its potential as a biomarker or therapeutic target in epithelial cancers and regenerative medicine.
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