| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GRAMD2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GRAMD2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GRAMD2A抗体的3篇参考文献示例(文献信息为虚拟模拟，仅供参考)：
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1. **文献名称**: *GRAMD2A modulates cellular cholesterol transport via ER-mitochondria interactions*
**作者**: Tanaka K, et al.
**摘要**: 本研究利用GRAMD2A特异性抗体进行免疫共沉淀和免疫荧光染色，揭示了GRAMD2A通过调控内质网与线粒体间的膜接触位点，影响胆固醇的细胞内转运。实验表明，敲低GRAMD2A会破坏脂质稳态，抗体验证了其与STARD3蛋白的相互作用。
2. **文献名称**: *A novel monoclonal antibody against GRAMD2A for functional studies in cancer cells*
**作者**: Müller S, et al.
**摘要**: 文章报道了一种针对GRAMD2A的单克隆抗体的开发，通过Western blot和流式细胞术验证其特异性。该抗体用于检测乳腺癌细胞中GRAMD2A的表达水平，发现其高表达与细胞迁移和侵袭能力增强相关。
3. **文献名称**: *GRAMD2A regulates lysosomal lipid sensing through mTORC1 signaling*
**作者**: Park J, et al.
**摘要**: 通过免疫组化(使用GRAMD2A抗体)和基因编辑技术，研究发现GRAMD2A通过感知溶酶体胆固醇水平调控mTORC1活性。抗体在组织样本中检测到GRAMD2A在肝脏和脂肪组织中的高表达，提示其代谢调控功能。
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注：以上文献为模拟示例，实际引用需查询PubMed、Google Scholar等数据库获取真实研究。若需具体文章，建议使用关键词“GRAMD2A antibody”或“GRAMD2A function”进行检索。
GRAMD2A (GRAM Domain Containing 2A) is a protein encoded by the GRAMD2A gene, belonging to the GRAMD family known for their roles in lipid metabolism and membrane-associated processes. This protein contains a GRAM domain, which is implicated in membrane binding and lipid sensing, and a transmembrane domain, suggesting its involvement in intracellular cholesterol transport and homeostasis. GRAMD2A is thought to interact with other lipid regulators, such as STARD3/GRAMD1A, to facilitate non-vesicular cholesterol movement within cells, particularly between the endoplasmic reticulum and plasma membrane.
Antibodies targeting GRAMD2A are essential tools for studying its expression, localization, and function in cellular cholesterol dynamics. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides derived from specific regions of the GRAMD2A protein (e.g., C-terminal or N-terminal epitopes). They are validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation, often with verification via knockout (KO) controls to ensure specificity.
Research utilizing GRAMD2A antibodies has linked the protein to pathologies like cancer, neurodegenerative diseases, and atherosclerosis, where cholesterol dysregulation plays a critical role. Commercial suppliers, including companies like Abcam and Novus Biologicals, provide these antibodies, often accompanied by validation data. Ongoing studies aim to clarify GRAMD2A's precise mechanisms, highlighting its potential as a therapeutic target in lipid-related disorders.
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