首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human intestinal fatty acid binding protein,iFABP ELISA Kit

  • 中文名： 人肠脂肪酸结合蛋白(iFABP)ELISA Kit
货号: IPD-E08024h
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    人肠脂肪酸结合蛋白(iFABP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FABP2含量。FABP2即脂肪酸结合蛋白2，主要在小肠表达。它参与长链脂肪酸摄取、转运和代谢调节。相关研究围绕其结合脂肪酸的机制，以及在脂肪代谢异常、胰岛素抵抗等疾病发生发展中的作用展开，有望成为代谢相关疾病治疗的潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.039 ng/mL。科研人员可通过定量评估肠道疾病模型（如小鼠肠缺血再灌注、细胞损伤实验）中iFABP的动态变化，或用于探究药物、营养素对肠道屏障功能的调控作用，为肠道病理机制研究及干预策略开发提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    FABP 2 ELISA Kit; FABP2 ELISA Kit; FABPI ELISA Kit; FABPI_HUMAN ELISA Kit; Fatty acid binding protein 2 intestinal ELISA Kit; Fatty acid binding protein ELISA Kit; Fatty acid binding protein intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Fatty acid-binding protein ELISA Kit; I FABP ELISA Kit; I-FABP ELISA Kit; IFABP ELISA Kit; intestinal ELISA Kit; intestinal FABP ELISA Kit; Intestinal fatty acid binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; MGC133132 ELISA Kit; OTTHUMP00000163925 ELISA Kit
  • 缩写：
    FABP2
  • Uniprot No.：
    P12104
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    85-105


    1:2

    Average %

    95


    Range %

    91-105


    1:4

    Average %

    99


    Range %

    92-107


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-103


    EDTA plasma (n=4)

    99

    90-108


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.412

    2.534

    2.473

    2.335


    5

    1.695

    1.667

    1.681

    1.543


    2.5

    1.117

    1.068

    1.093

    0.955


    1.25

    0.696

    0.659

    0.677

    0.539


    0.625

    0.419

    0.421

    0.420

    0.282


    0.312

    0.320

    0.333

    0.326

    0.189


    0.156

    0.202

    0.194

    0.198

    0.060


    0

    0.141

    0.135

    0.138



  • 本试剂盒所含材料：


    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human FABP2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled FABP2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate

      • 货期：
        3-5 working days

      客户数据及评论

      折叠内容

      大包装询价

      ×