| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ANOP3; MCOPS3 |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SOX2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SOX2抗体的3-4篇参考文献，按文献名称、作者和摘要内容概括列举：
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1. **文献名称**：*SOX2 is a persistent marker of pluripotency in the mouse embryonic stem cells*
**作者**：Avilion, A.A. 等 (2003. *Nature*)
**摘要**：该研究首次证实SOX2抗体在胚胎干细胞中的特异性标记作用，发现SOX2蛋白对维持干细胞多能性至关重要，并通过免疫染色和Western blot验证了抗体的可靠性。
2. **文献名称**：*SOX2 expression in glioblastoma: a marker of cancer stem cells and prognostic factor*
**作者**：Schmidt, C.S. 等 (2014. *Cancer Cell*)
**摘要**：利用SOX2抗体进行免疫组化分析胶质母细胞瘤样本，发现SOX2高表达与肿瘤干细胞特性相关，且与患者预后不良显著相关。
3. **文献名称**：*Antibody validation for specificity: SOX2 antibodies in developmental studies*
**作者**：Bergsland, M. 等 (2006. *Developmental Biology*)
**摘要**：系统评估了多种SOX2抗体的特异性，发现部分抗体在神经发育模型中存在交叉反应，强调选择高特异性抗体对研究的重要性。
4. **文献名称**：*SOX2 as a diagnostic marker in solid tumors*
**作者**：Karachi, A. 等 (2018. *Clinical Cancer Research*)
**摘要**：通过大规模临床样本分析，验证SOX2抗体在多种实体瘤(如肺癌、脑瘤)中的诊断价值，并探讨其作为治疗靶点的潜力。
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以上文献涵盖了SOX2抗体在基础研究、癌症机制、抗体验证及临床诊断中的应用，均为领域内代表性研究。
**Background of SOX2 Antibody**
SOX2 (SRY-box transcription factor 2) is a member of the SOX family of transcription factors characterized by a high-mobility group (HMG) DNA-binding domain. It plays a critical role in maintaining pluripotency in embryonic stem cells and regulating self-renewal and differentiation in various progenitor cells. SOX2 is essential during early embryonic development, particularly in neural tube formation, lens development, and maintaining the identity of neural stem cells. Dysregulation of SOX2 expression is implicated in developmental disorders, such as anophthalmia/microphthalmia, and cancers, including lung, breast, and glioblastoma, where it often promotes tumorigenesis and stem-like properties.
SOX2 antibodies are immunological tools designed to detect and quantify SOX2 protein levels in research and diagnostic applications. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to study SOX2's expression patterns, localization, and function in cellular and tissue contexts. Both monoclonal and polyclonal SOX2 antibodies are available, with specificity validated across human, mouse, and rat samples.
In cancer research, SOX2 antibodies help identify tumor cells with stem-like characteristics, aiding in understanding therapeutic resistance mechanisms. In regenerative medicine, they assist in tracking pluripotent stem cell differentiation. Proper validation of SOX2 antibodies is crucial to avoid cross-reactivity with other SOX family members (e.g., SOX1. SOX3) and ensure accurate interpretation of experimental results.
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