首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠叶酸(FA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的FA含量。试剂盒检测范围为0.195 ng/mL-50 ng/mL，灵敏度为0.195 ng/mL。适用于科研场景下的小鼠营养学研究、叶酸缺乏模型评估、代谢性疾病干预实验等，可精准追踪体内叶酸动态变化，为探索叶酸在生长发育、氧化应激或免疫调节中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：FA
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.195 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 90-105 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 83-96 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 84-99 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 85-99 | ||||||
回收率：
| The recovery of mouse FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 87-100 | |||||
| EDTA plasma (n=4) | 95 | 89-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 500.3210.3320.327 12.50.5120.5370.525 3.120.8260.8510.839 0.781.1751.1531.164 0.1951.6551.6321.644 01.9851.9481.967 | |||||||
货期：3-5 working days
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