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产品描述：人叶酸(FA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的FA含量。试剂盒检测范围为12.5 pg/mL-200 pg/mL，灵敏度为6.25 pg/mL。适用于营养学研究、代谢性疾病机制探索、药物干预效果评估等科研领域，特别在叶酸代谢与妊娠健康、血液系统疾病关联性研究中具有应用价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：FA
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：12.5 pg/mL-200 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:80 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:160 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-105 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.0930.0900.092 1000.2270.2080.218 500.3780.3670.373 250.6180.6370.628 12.50.9861.0030.995 01.4851.4291.457 | ||||||
本试剂盒所含材料：
A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human FA antibody.
Standard (10 x concentrate)(1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
HRP-conjugated FA (100 x concentrate) (1 x 6oμl)
HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated FA antibody solution.
Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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