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Canine Folic acid,FA ELISA Kit

  • 中文名： 犬叶酸(FA)ELISA Kit
货号: IPD-E08759c
Price： ￥3000
数量：
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产品详情

  • 产品描述：
    犬叶酸(FA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的FA含量。试剂盒检测范围为0.195 ng/mL-50 ng/mL，灵敏度为0.195 ng/mL。适用于科研领域中对犬类营养代谢研究、疾病模型叶酸水平监测（如消化吸收障碍或血液系统异常）以及兽药/饲料干预效果评估等场景，为动物医学、比较生理学等研究提供可靠的叶酸定量工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    FA
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.195 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of Canine FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    97


    Range %

    90-105


    1:2

    Average %

    89


    Range %

    83-96


    1:4

    Average %

    92


    Range %

    84-99


    1:8

    Average %

    91


    Range %

    85-99


  • 回收率：

    The recovery of Canine FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    87-100


    EDTA plasma (n=4)

    95

    89-99


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average



    50

    0.311

    0.302

    0.307



    12.5

    0.524

    0.519

    0.522



    3.12

    0.846

    0.857

    0.852



    0.78

    1.188

    1.203

    1.196



    0.195

    1.642

    1.628

    1.635



    0

    2.056

    1.997

    2.027




  • 货期：
    3-5 working days

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