| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HMMS; IRXB2; IRX-2a |
| WB Predicted band size | 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human IRX5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于IRX5抗体的参考文献示例(均为虚构，仅用于示例用途)：
1. **文献名称**：*IRX5 regulates ventricular action potential duration through transcriptional control of potassium channels*
**作者**：Smith J. et al. (2015)
**摘要**：本研究通过免疫组化和小鼠模型，利用IRX5抗体揭示了IRX5在心室肌细胞中的梯度表达模式，发现其通过调控Kv4.2等钾离子通道基因影响心脏电生理特性。
2. **文献名称**：*IRX5 promotes glioma progression by activating the Wnt/β-catenin pathway*
**作者**：Zhang L. et al. (2018)
**摘要**：通过Western blot和免疫荧光实验，结合IRX5抗体，研究者证实IRX5在胶质瘤中高表达，并通过激活Wnt通路促进肿瘤侵袭和干细胞特性。
3. **文献名称**：*Developmental role of IRX5 in limb patterning and skeletal formation*
**作者**：Lee S. et al. (2012)
**摘要**：利用IRX5抗体进行胚胎组织染色，发现IRX5在小鼠肢体发育中调控骨骼形成相关基因(如Sox9)，揭示了其在前-后轴模式决定中的关键作用。
4. **文献名称**：*Chromatin profiling identifies IRX5 as a novel oncogenic driver in breast cancer*
**作者**：Gupta R. et al. (2020)
**摘要**：通过ChIP-seq结合IRX5抗体，研究者发现IRX5在乳腺癌中与染色质重塑复合物结合，促进促癌基因(如MYC)的转录，提示其作为潜在治疗靶点。
(注：以上文献及作者为虚构示例，如需真实文献建议通过PubMed/Google Scholar检索IRX5及相关关键词。)
The IRX5 antibody is a research tool designed to detect and study the Iroquois homeobox protein 5 (IRX5), a transcription factor belonging to the Iroquois (Irx) gene family. This family, conserved across vertebrates, plays critical roles in embryonic development, particularly in patterning neural, cardiac, and limb tissues. IRX5 is involved in regulating gene expression networks during organogenesis, with notable functions in heart development, where it influences chamber-specific differentiation, and in the nervous system, where it contributes to neuronal subtype specification. Dysregulation of IRX5 has been linked to congenital defects, including cardiac abnormalities, and its aberrant expression is implicated in cancers such as melanoma and colorectal carcinoma, where it may act as an oncogene or tumor suppressor depending on context.
The IRX5 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to visualize protein localization, expression levels, and interactions in cellular or tissue samples. Its validation typically includes testing for specificity via knockout controls or siRNA knockdown. Researchers employ this antibody to explore IRX5's role in developmental biology, disease mechanisms, and potential therapeutic targets. As Irx proteins often exhibit functional redundancy, precise detection of IRX5 is crucial for disentangling its unique contributions within complex regulatory pathways. Commercial availability from multiple biotech vendors has facilitated its adoption in both basic and translational research settings.
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