| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ITGAV; MSK8; VNRA; VTNR |
| Entrez GeneID | 3685 |
| clone | 3H1A5 |
| WB Predicted band size | 116kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD51 (AA: extra 31-189) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于IP6K1抗体的3篇示例参考文献(内容为模拟，仅供参考)：
1. **"Characterization of IP6K1-specific antibodies for detection of inositol hexakisphosphate kinase activity"**
- 作者：Saiardi, A. 等
- 摘要：研究开发并验证了针对IP6K1的多克隆抗体，证实其特异性用于Western blot和免疫沉淀实验，揭示了IP6K1在细胞内的酶活性调控机制。
2. **"IP6K1 regulates apoptosis via its interaction with Bcl-2 family proteins"**
- 作者：Bhandari, R. 等
- 摘要：通过定制IP6K1抗体，研究发现IP6K1通过磷酸化作用调控Bcl-2蛋白，影响线粒体途径的细胞凋亡，抗体特异性经敲除细胞系验证。
3. **"Role of IP6K1 in cancer metastasis: Insights from antibody-mediated inhibition studies"**
- 作者：Morrison, B.H. 等
- 摘要：利用IP6K1单克隆抗体阻断其功能，发现IP6K1通过调控细胞迁移相关信号通路促进肿瘤转移，抗体在动物模型中显示潜在治疗价值。
**备注**：以上文献信息为示例，实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“IP6K1 antibody”、“IP6K1 kinase antibody”结合筛选近十年的研究获取准确信息。
The IP6K1 antibody is a crucial tool for studying inositol hexakisphosphate kinase 1 (IP6K1), an enzyme involved in synthesizing inositol pyrophosphates like 5-diphosphoinositol pentakisphosphate (5-IP7). IP6K1 belongs to the IP6K family (IP6K1-3), which regulates cellular processes such as energy metabolism, apoptosis, insulin secretion, and chromatin remodeling. IP6K1 catalyzes the phosphorylation of IP6 to 5-IP7. a high-energy signaling molecule influencing vesicular trafficking, telomere maintenance, and stress responses. Dysregulation of IP6K1 is linked to metabolic disorders, neurodegenerative diseases, and cancer, making it a therapeutic target.
IP6K1 antibodies are designed to detect endogenous IP6K1 protein levels, localization, and expression patterns in various tissues or cell lines. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Specificity is validated through knockout controls or siRNA-mediated knockdown. These antibodies often target conserved regions, such as the catalytic domain or unique C-/N-terminal sequences, to distinguish IP6K1 from homologs (e.g., IP6K2/3). Research applications include exploring IP6K1's role in metabolic pathways (e.g., diabetes), cancer cell proliferation, and neurological functions, aiding in mechanistic studies and drug discovery.
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