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Rabbit Polyclonal IST1 Antibody

  • 中文名: IST1抗体
  • 别    名: OLC1
货号: IPDX06768
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/40-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesOLC1
WB Predicted band size40 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human IST1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于IST1抗体的3篇参考文献及其简要摘要:

1. **文献名称**: "The role of IST1 and CHMP1B in the regulation of ESCRT-III assembly and abscission"

**作者**: Agromayor, M., et al.

**摘要**: 研究揭示了IST1与ESCRT-III复合体成员CHMP1B的相互作用,通过调控细胞分裂末期膜切割(abscission)的时序,证明IST1抗体可用于检测其在胞质分裂中的动态定位。

2. **文献名称**: "IST1 regulates ESCRT-III assembly via its conserved C-terminal domain"

**作者**: Bajorek, M., et al.

**摘要**: 通过结构分析和体外重组实验,阐明IST1通过C端结构域促进ESCRT-III复合体的组装,研究利用IST1抗体验证其在膜重塑过程中的功能。

3. **文献名称**: "Cooperative binding of IST1 and VPS4 to ESCRT-III filaments drives membrane remodeling"

**作者**: McCullough, J., et al.

**摘要**: 结合冷冻电镜和生化实验,证明IST1与ATP酶VPS4协同作用解聚ESCRT-III聚合物,IST1抗体用于追踪其在病毒出芽和细胞分裂中的活性。

4. **文献名称**: "IST1 modulates membrane remodeling in autophagy and endosomal sorting"

**作者**: Guizetti, J., et al.

**摘要**: 研究显示IST1通过调控ESCRT-III的膜结合能力参与自噬小体形成和内体分选,使用IST1抗体揭示其在不同膜动态过程中的亚细胞定位。

(注:以上文献为模拟示例,实际引用请核对真实数据库及原文。)

背景信息

The IST1 antibody targets the IST1 (Increased Sodium Tolerance 1 homolog) protein, a component of the endosomal sorting complex required for transport (ESCRT) machinery. IST1 plays a critical role in membrane remodeling processes, such as cytokinesis, multivesicular body (MVB) formation, and viral budding, by facilitating the assembly and disassembly of ESCRT-III polymers. It interacts with other ESCRT components, including CHMP1B and VPS4. to regulate membrane scission and cargo sorting. IST1 antibodies are widely used in research to investigate cellular division, intracellular trafficking, and organelle biogenesis. They are applied in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to study IST1 expression, localization, and interactions. Dysregulation of IST1 or ESCRT pathways is linked to neurological disorders, cancer, and infectious diseases, making this antibody a valuable tool for exploring disease mechanisms. Recent studies also highlight its role in autophagy and lysosomal function, expanding its relevance in cellular homeostasis research.

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