| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ISM; Isthmin; C20orf82; bA149I18.1; dJ1077I2.1 |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ISM1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ISM1抗体的3篇代表性文献的简要信息(注：部分内容基于假设性研究框架整理)：
1. **文献名称**: "Isthmin-1 (ISM1) suppresses tumor growth through immunomodulation and acts as a novel antibody-drug conjugate target"
**作者**: Chen, L., et al.
**摘要**: 本研究首次开发了靶向ISM1的单克隆抗体，证实其通过激活自然杀伤细胞抑制小鼠模型中黑色素瘤的生长。抗体偶联药物(ADC)实验显示显著增强肿瘤细胞杀伤效果。
2. **文献名称**: "Structural characterization of ISM1 antibodies reveals dual mechanism in angiogenesis inhibition"
**作者**: Watanabe, K., et al.
**摘要**: 通过冷冻电镜解析ISM1抗体-抗原复合物结构，发现抗体通过阻断ISM1与αVβ3整合素结合，同时诱导血管内皮细胞凋亡，为抗血管生成治疗提供新策略。
3. **文献名称**: "ISM1 as a serum biomarker: Development of a quantitative ELISA for early cancer detection"
**作者**: Gupta, R., et al.
**摘要**: 团队研发了高灵敏度夹心法ELISA检测试剂盒，验证ISM1在胰腺癌患者血清中显著高表达(AUC=0.92)，抗体对在50例临床样本中显示出优异诊断一致性。
注：以上内容为领域研究方向的模拟示例，实际文献需通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Web of Science等数据库检索确认。建议使用关键词"ISM1 antibody"+"therapeutic"或"diagnostic"获取最新研究进展。
The ISM1 (Isthmin 1) antibody targets a secreted protein encoded by the *ISM1* gene, initially identified in zebrafish for its role in embryonic development, particularly at the midbrain-hindbrain boundary. In humans, ISM1 is implicated in diverse biological processes, including apoptosis, angiogenesis, glucose metabolism, and immune regulation. Structurally, ISM1 contains a thrombospondin type-1 repeat (TSR) domain and two scavenger receptor cysteine-rich (SRCR) domains, suggesting roles in extracellular signaling and protein interactions. Dysregulation of ISM1 has been linked to cancer, metabolic disorders, and inflammatory diseases, highlighting its therapeutic potential.
ISM1 antibodies are essential tools for detecting ISM1 expression and localization in tissues or cells via techniques like Western blot, immunohistochemistry, and ELISA. They also enable functional studies, such as blocking ISM1 activity to explore its physiological or pathological roles. These antibodies are typically developed in hosts like rabbits or mice, with specificity validated through knockout controls or peptide blocking assays. Recent studies emphasize ISM1's dual role in cancer—acting as a tumor suppressor by inhibiting angiogenesis or inducing apoptosis in certain contexts, while potentially promoting metastasis in others. Its metabolic functions, including interactions with insulin signaling pathways, further underscore its relevance in diabetes and obesity research.
Ongoing research aims to clarify ISM1's mechanisms and therapeutic applications, driving demand for high-quality antibodies to support biomedical discoveries.
×
