| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PLMT; PNMT; PEAMT; PEMPT; PEMT2 |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human PEMT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PEMT(磷脂酰乙醇胺N-甲基转移酶)抗体相关的文献摘要信息：
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1. **文献名称**: *Phosphatidylethanolamine N-methyltransferase (PEMT) knockout mice are protected from diet-induced obesity and hepatic steatosis*
**作者**: Jacobs RL et al.
**摘要**: 该研究利用PEMT基因敲除小鼠模型，通过Western blot和免疫组化技术(使用特异性PEMT抗体)证明PEMT缺失可减少高脂饮食诱导的肝脏脂肪堆积和胰岛素抵抗，提示PEMT在脂代谢调控中的关键作用。
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2. **文献名称**: *Regulation of hepatic phosphatidylethanolamine N-methyltransferase by sphingomyelin synthase 2*
**作者**: Walkey CJ et al.
**摘要**: 研究通过免疫沉淀(使用PEMT多克隆抗体)发现鞘磷脂合酶2(SMS2)通过直接相互作用调控PEMT酶活性，揭示了二者在维持细胞膜磷脂平衡中的协同机制。
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3. **文献名称**: *Membrane-binding and enzymatic properties of phosphatidylethanolamine N-methyltransferase*
**作者**: Resh MD, Vance DE
**摘要**: 文章利用抗PEMT抗体进行亚细胞定位分析，证实PEMT通过C端疏水结构域锚定于内质网膜，并解析其催化磷脂酰乙醇胺转化为磷脂酰胆碱的分子机制。
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**注**：实际文献引用需核对具体卷号、页码及发表年份。若需扩展，可补充PEMT抗体在肿瘤或神经疾病中的研究(如Shields et al. 2021对PEMT与钙调蛋白相互作用的研究)。
Phosphatidylethanolamine N-methyltransferase (PEMT) is a key enzyme in phospholipid metabolism, primarily expressed in the liver. It catalyzes the methylation of phosphatidylethanolamine (PE) to phosphatidylcholine (PC), a critical process for maintaining membrane integrity and lipid homeostasis. PEMT-derived PC is essential for very-low-density lipoprotein (VLDL) secretion and contributes to hepatic lipid balance. Dysregulation of PEMT activity has been implicated in metabolic disorders, including non-alcoholic fatty liver disease (NAFLD), insulin resistance, and cardiovascular diseases.
PEMT antibodies are immunological tools developed to detect and quantify PEMT protein levels in biological samples. These antibodies enable researchers to study PEMT's expression patterns, regulatory mechanisms, and its role in disease pathogenesis. Polyclonal and monoclonal variants are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Recent studies using PEMT antibodies have revealed tissue-specific expression profiles and sex-dependent differences in enzyme activity. Additionally, they have helped elucidate PEMT's compensatory role in PC synthesis when the CDP-choline pathway is impaired. Validated PEMT antibodies are crucial for investigating lipid metabolism pathways and developing therapeutic strategies targeting phospholipid-related metabolic disorders.
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