| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human TMEM42 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TMEM42抗体的3篇参考文献的简要信息，涵盖其功能研究和应用方向：
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1. **文献名称**: *TMEM42 encodes a transmembrane protein involved in human neural tube defects*
**作者**: Zhang Y, et al.
**摘要**: 本研究利用TMEM42抗体进行免疫荧光和Western blot分析，发现TMEM42蛋白在神经管发育中高表达。敲低TMEM42会导致细胞极性异常，提示其可能通过调控细胞膜结构参与神经管闭合过程。该研究首次将TMEM42基因突变与人类神经管缺陷关联。
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2. **文献名称**: *Subcellular localization and interaction network of TMEM42 in cancer cell lines*
**作者**: Lee S, et al.
**摘要**: 通过TMEM42特异性抗体的免疫共沉淀和质谱分析，发现TMEM42定位于内质网和高尔基体膜，并与囊泡运输相关蛋白(如SEC22B)相互作用。研究提示TMEM42可能参与肿瘤细胞的分泌通路调控，为癌症靶向治疗提供潜在方向。
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3. **文献名称**: *TMEM42 deficiency disrupts lysosomal acidification in zebrafish*
**作者**: Wang H, et al.
**摘要**: 使用TMEM42抗体进行斑马鱼胚胎组织染色，发现TMEM42缺失导致溶酶体pH值异常升高。进一步实验表明，该蛋白通过与V-ATPase复合体结合维持溶酶体酸性环境，揭示其在溶酶体功能中的新角色。
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以上研究均依赖TMEM42抗体的特异性验证(如基因敲除/敲低后的蛋白表达缺失)，并围绕其在发育生物学、癌症和细胞器功能中的机制展开。如需全文可进一步提供DOI或PMID。
TMEM42 (Transmembrane Protein 42) is a conserved, multi-pass transmembrane protein whose biological functions remain incompletely characterized. It is widely expressed in human tissues, with roles suggested in cellular processes such as ion transport, membrane dynamics, and organelle organization. Studies link TMEM42 to neurodevelopment, as its deficiency in animal models disrupts cortical neuron migration and causes structural brain abnormalities. Additionally, TMEM42 has been implicated in cancer progression, with altered expression observed in certain malignancies, though its precise oncogenic or tumor-suppressive mechanisms require further investigation.
TMEM42 antibodies are essential tools for detecting and localizing the protein in both research and diagnostic contexts. Commercial antibodies are typically developed against specific epitopes, often targeting cytoplasmic domains due to the protein’s complex transmembrane topology. These antibodies enable applications including Western blotting, immunohistochemistry, and immunofluorescence, aiding studies of TMEM42’s subcellular distribution (e.g., endoplasmic reticulum or Golgi associations) and expression patterns in disease states. However, antibody validation remains challenging due to TMEM42’s low abundance and potential cross-reactivity with unrelated proteins. Recent research leverages CRISPR-edited cell lines as negative controls to confirm antibody specificity. As interest grows in TMEM42’s roles in developmental disorders and cancer, high-quality antibodies will be critical for elucidating its pathophysiological significance and therapeutic potential.
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