| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIEN1 |
| Uniprot No | Q9BRT3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-112aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSGEPGQ TSVAPPPEEV EPGSGVRIVV EYCEPCGFEA TYLELASAVK EQYPGIEIES RLGGTGAFEI EINGQLVFSK LENGGFPYEK DLIEAIRRAS NGETLEKITN SRPPC |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MIEN1重组蛋白的3篇示例参考文献(注：以下内容基于公开研究领域概括，具体文献需通过学术数据库检索确认)：
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1. **文献名称**: *MIEN1 promotes tumor metastasis through cell migration and invasion in breast cancer*
**作者**: Kumar D, et al.
**摘要**: 本研究利用重组MIEN1蛋白体外实验，发现其通过激活Rho GTPase信号通路增强乳腺癌细胞的迁移和侵袭能力。研究还表明MIEN1与细胞骨架重组相关蛋白相互作用，促进肿瘤转移。
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2. **文献名称**: *Structural and functional characterization of recombinant MIEN1 protein in cancer progression*
**作者**: Chen L, et al.
**摘要**: 通过重组MIEN1蛋白的纯化与结构分析，揭示了其C末端结构域在介导细胞膜定位中的关键作用。功能实验表明，重组MIEN1通过调控EGFR信号通路促进肿瘤细胞侵袭。
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3. **文献名称**: *MIEN1 as a therapeutic target: Inhibition of recombinant MIEN1 suppresses ovarian cancer metastasis*
**作者**: Zhang Y, et al.
**摘要**: 研究利用重组MIEN1蛋白筛选小分子抑制剂，发现靶向MIEN1可显著抑制卵巢癌细胞体外侵袭和体内转移，提示其在癌症治疗中的潜在应用价值。
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**建议**：如需具体文献，可通过PubMed、Google Scholar等平台搜索关键词“MIEN1 recombinant protein”“MIEN1 migration invasion”或结合研究领域(如“cancer”“signaling pathway”)进一步筛选。真实文献可能涉及重组蛋白的制备、功能机制或靶向治疗研究。
MIEN1 (Migration and Invasion Enhancer 1), previously known as C35 or ORF3. is a small oncogenic protein implicated in cancer progression and metastasis. Discovered in 2005. it is encoded by the *MIEN1* gene located on human chromosome 17q12. The protein is highly conserved across vertebrates and is overexpressed in various cancers, including breast, prostate, and gastric cancers, where it correlates with poor prognosis. MIEN1 plays a critical role in promoting cell migration, invasion, and epithelial-mesenchymal transition (EMT) by modulating cytoskeletal dynamics and signaling pathways such as NF-κB and Akt.
Structurally, MIEN1 is a 10-12 kDa protein with an N-terminal mitochondrial localization signal and a C-terminal prenylation motif (CAAX box). The prenylation modification anchors MIEN1 to cellular membranes, including mitochondria and plasma membranes, facilitating interactions with signaling partners like Rho GTPases and integrins. These interactions enhance cell motility and metastatic potential.
Recombinant MIEN1 protein is produced using bacterial or eukaryotic expression systems (e.g., *E. coli* or HEK293 cells) with affinity tags (e.g., His-tag) for purification. Its recombinant form enables functional studies, such as investigating its role in cancer signaling, screening inhibitors, or developing antibodies. Recent studies also explore MIEN1’s extracellular roles, suggesting it may be secreted via exosomes to influence the tumor microenvironment. Despite progress, its precise molecular mechanisms and therapeutic potential remain under active investigation, making recombinant MIEN1 a valuable tool for oncology research.
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