Rabbit Polyclonal STAT1 Antibody

The STAT1 (Signal Transducer and Activator of Transcription 1) antibody is a crucial tool for studying the JAK-STAT signaling pathway, which mediates cellular responses to cytokines, interferons (IFNs), and growth factors. STAT1 is a transcription factor activated by phosphorylation following receptor-ligand binding, enabling its dimerization, nuclear translocation, and regulation of target genes involved in immunity, inflammation, and apoptosis. Research applications of STAT1 antibodies include Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect STAT1 expression, localization, or post-translational modifications (e.g., phosphorylation at Tyr701 or Ser727). These antibodies help distinguish between inactive monomeric STAT1. phosphorylated active dimers, and isoform variants (STAT1α and STAT1β). Dysregulation of STAT1 is linked to immune disorders, cancer, and infectious diseases. For instance, STAT1 gain-of-function mutations cause autoimmune diseases, while loss-of-function mutations impair antiviral responses. In cancer, STAT1 exhibits dual roles: it promotes tumor suppression by enhancing IFN-mediated immune surveillance but may also drive therapy resistance in certain contexts. Antibodies targeting specific STAT1 epitopes are essential for elucidating its context-dependent roles, validating its interaction partners, and developing therapeutic strategies targeting JAK-STAT signaling. Commercial STAT1 antibodies are typically validated for cross-reactivity across human, mouse, and rat samples, with controls to ensure specificity given structural similarities among STAT family proteins.