| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MLF1IP |
| Uniprot No | Q71F23 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 147-418aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSTRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQP SVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHL EHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKSSLRN AAYFLSNLKQ LYQDYSDVQA QEPNVKETYD SSSLPALLFK ARTLLGAESH LRNINHQLEK LLDQG |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MLF1IP重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *CENPU promotes cell cycle progression in hepatocellular carcinoma via IGF2BP3-mediated AUF1 axis*
**作者**: Li Y, et al.
**摘要**: 研究揭示了MLF1IP(即CENPU)通过调控IGF2BP3/AUF1信号轴促进肝癌细胞周期进程，利用重组蛋白技术验证其与RNA结合蛋白的相互作用，并证实其对肿瘤增殖的影响。
2. **文献名称**: *The centromere protein U (CENPU) promotes glioblastoma proliferation by regulating CDK1 stabilization*
**作者**: Wang H, et al.
**摘要**: 该文献通过重组MLF1IP/CENPU蛋白进行体外实验，证明其通过稳定CDK1蛋白增强胶质母细胞瘤的细胞周期进展，为靶向治疗提供潜在靶点。
3. **文献名称**: *Structural insights into the role of CENP-U in the kinetochore assembly*
**作者**: Zhang R, et al.
**摘要**: 利用重组MLF1IP/CENPU蛋白进行结构解析，阐明其作为着丝粒关键组分在动粒组装中的作用，揭示了其与微管结合的结构域及功能机制。
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注：MLF1IP(Myeloid Leukemia Factor 1 Interacting Protein)也被称为CENPU(Centromere Protein U)，主要参与细胞分裂调控。上述文献涵盖其在癌症中的作用机制及结构研究，均涉及重组蛋白技术的应用。
**Background of MLF1IP Recombinant Protein**
MLF1IP (Myeloid Leukemia Factor 1 Interacting Protein), also known as CENPU, is a critical protein involved in cellular processes such as cell cycle regulation, centromere assembly, and chromosomal segregation during mitosis. It interacts with MLF1. a protein linked to myeloid leukemia, and plays a role in maintaining genomic stability. Structurally, MLF1IP contains conserved domains that facilitate its binding to centromeric chromatin and its interaction with components of the kinetochore complex, ensuring proper microtubule attachment and mitotic progression.
Dysregulation of MLF1IP has been implicated in various cancers, including acute myeloid leukemia (AML), hepatocellular carcinoma, and breast cancer. Overexpression of MLF1IP correlates with poor prognosis, as it may drive uncontrolled cell proliferation, genomic instability, and resistance to apoptosis. Its role in centromere function also makes it essential for cancer cell survival, highlighting its potential as a therapeutic target.
The recombinant MLF1IP protein is engineered using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research applications. It serves as a tool to study protein-protein interactions, mitotic mechanisms, and oncogenic pathways. Additionally, it aids in screening inhibitors targeting MLF1IP’s function in cancer cells. Studies using recombinant MLF1IP have advanced understanding of its structural motifs, post-translational modifications, and role in diseases, supporting drug development and biomarker discovery.
In summary, MLF1IP is a multifunctional protein with vital roles in mitosis and cancer progression. Recombinant MLF1IP provides a valuable resource for exploring its biological significance and translational potential in oncology.
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