| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/2000 | Human,Mouse,Rat |
| Aliases | 5-HT2B, 5-HT(2B) |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human HTR2B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HTR2B抗体的3篇代表性文献(内容为虚构示例，仅供参考)：
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1. **文献名称**：*Development of a High-Specificity Antibody for Human 5-HT2B Receptor Detection*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种针对人HTR2B受体胞外域的单克隆抗体，通过免疫印迹和免疫组化验证其特异性，并应用于脑组织及心肌细胞的受体定位研究。
2. **文献名称**：*HTR2B Expression in Pulmonary Hypertension: Role of Serotonin Signaling*
**作者**：Lee C, et al.
**摘要**：利用HTR2B抗体分析肺动脉高压患者肺血管平滑肌细胞的受体表达上调，揭示其通过MAPK通路促进细胞增殖的分子机制。
3. **文献名称**：*5-HT2B Receptor Antibody Validation for Neuronal Circuit Mapping*
**作者**：Garcia R, et al.
**摘要**：通过敲除小鼠模型验证HTR2B抗体的特异性，结合荧光标记技术解析该受体在前额叶皮层神经元中的分布及其与抑郁行为的关联。
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(注：以上文献为示例，实际引用需查询真实数据库如PubMed。)
The 5-hydroxytryptamine receptor 2B (HTR2B), a G protein-coupled receptor (GPCR), binds serotonin (5-HT) and mediates various physiological processes, including mood regulation, cardiovascular function, and gastrointestinal activity. Primarily expressed in the central nervous system, heart, lungs, and gut, HTR2B activates intracellular signaling pathways (e.g., phospholipase C) upon ligand binding, influencing cell proliferation, neurotransmission, and vascular tone. Its aberrant activity is linked to pathologies such as pulmonary hypertension, cardiac valvulopathy (notably associated with fenfluramine-derived drugs), and neuropsychiatric disorders.
HTR2B-specific antibodies are essential tools for studying receptor localization, expression levels, and signaling mechanisms in research. They enable techniques like Western blotting, immunohistochemistry, and flow cytometry to explore HTR2B's role in disease models or drug responses. Due to structural similarities among 5-HT receptor subtypes, antibody specificity is critical to avoid cross-reactivity. Recent studies also highlight HTR2B's potential as a therapeutic target, driving demand for validated antibodies in preclinical drug development. However, limited commercial availability and variable validation standards pose challenges, emphasizing the need for rigorous antibody characterization in experimental workflows.
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