| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CBX6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及CBX6抗体的研究文献摘要概览：
1. **《CBX6 is a critical regulator of pluripotency in human embryonic stem cells》**
- 作者：Zhao Y. et al. (2018)
- 摘要：研究通过免疫共沉淀(使用CBX6特异性抗体)发现CBX6通过调控PRC1复合体维持人胚胎干细胞多能性，敲低CBX6导致分化相关基因异常激活。
2. **《The Polycomb protein CBX6 promotes pancreatic cancer progression through EMT regulation》**
- 作者：Wang L. et al. (2020)
- 摘要：利用CBX6抗体进行免疫组化和Western blot，揭示CBX6在胰腺癌中高表达，通过促进上皮间质转化(EMT)驱动肿瘤侵袭转移。
3. **《Antibody-based profiling of Chromobox family proteins in hepatocellular carcinoma》**
- 作者：Chen H. et al. (2019)
- 摘要：开发了针对CBX家族(含CBX6)的特异性抗体，结合组织芯片分析，发现CBX6表达与肝癌患者预后不良显著相关，提示其作为潜在生物标志物。
注：以上为基于领域知识的模拟文献概括，实际文献需通过PubMed/Google Scholar以关键词"CBX6 antibody"+"specific function"检索确认。若需具体文献，建议补充更详细的研究背景或应用场景。
The CBX6 antibody is a tool used to study the Chromobox homolog 6 (CBX6) protein, a member of the Polycomb Repressive Complex 1 (PRC1) family. PRC1 is crucial for maintaining gene silencing through chromatin modification, including monoubiquitination of histone H2A. CBX6. like other Chromobox proteins (CBX2. 4. 6. 7. 8), contributes to PRC1’s role in epigenetic regulation, cell differentiation, and development by binding to trimethylated histone H3 lysine 27 (H3K27me3), a mark deposited by Polycomb Repressive Complex 2 (PRC2). This interaction facilitates chromatin compaction and transcriptional repression.
CBX6 antibodies are widely utilized in research to investigate PRC1-mediated gene regulation in stem cell pluripotency, tissue development, and cancer. Studies suggest CBX6 has tissue-specific roles; for example, it is implicated in maintaining neuronal progenitor cell identity and may function as an oncogene or tumor suppressor in certain cancers. Antibodies targeting CBX6 (often rabbit or mouse monoclonal) are validated for applications like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Specificity is confirmed using knockout controls or siRNA-mediated knockdown.
Unlike other CBX proteins, CBX6 exhibits distinct expression patterns and functional redundancy within the PRC1 complex, making its selective detection critical for understanding its unique contributions to epigenetic dynamics. Research on CBX6 also explores its interplay with non-Polycomb proteins and potential therapeutic relevance in diseases linked to epigenetic dysregulation.
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