Rabbit Polyclonal CDKN1A Antibody

The CDKN1A antibody is a crucial tool in molecular and cellular biology research, targeting the cyclin-dependent kinase inhibitor 1A (CDKN1A), also known as p21 or Cip1/Waf1. Encoded by the CDKN1A gene, this protein regulates cell cycle progression by inhibiting cyclin-dependent kinases (CDKs), thereby inducing cell cycle arrest in response to DNA damage or stress. CDKN1A plays a pivotal role in mediating tumor suppression, senescence, and apoptosis, often acting downstream of p53. Antibodies against CDKN1A are widely used to detect and quantify p21 expression in techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry. They help researchers study cellular responses to genotoxic stress, chemotherapy, or radiation, as well as explore mechanisms underlying cancer, aging, and tissue regeneration. Specificity and validation are critical, as p21 exists in multiple isoforms and undergoes post-translational modifications. Researchers must select antibodies validated for their experimental model (e.g., human, mouse) and application. Commercially available CDKN1A antibodies vary in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and conjugate types, enabling flexibility in experimental design. Proper controls, such as knockout cell lines, are essential to confirm antibody specificity.